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Methodology: Indirect Fluorescent Antibody (IFA)
Edit Date: 11/4/2010
Components: Poliovirus Type 1, 2, and 3 Antibodies
Performed: Wednesday, Friday
Released: 2-7 days after setup at PeaceHealth Laboratories’ reference lab
CPT Code: 86658-90 x3
Specimen Collection Details
Collection: One 7.5 mL serum separator tube (SST).
Handling: Allow to clot, centrifuge and separate serum into a plastic vial. Refrigerate.
Stability: 48 hours ambient, 7 days refrigerated, or 2 months frozen.
Standard Volume: 1 mL serum
Minimum Volume: 0.5 mL serum
Transport: Refrigerated
Comments: Detection of poliovirus antibodies due to immunization or previous exposure to polio or other enteroviruses. Cross-reaction with other enteroviruses are known. Less than 2% of tested individuals have less than 1:8 titer. If an acute polio infection is suspected, paired sera samples, at least three weeks apart, would demonstrate at minimum, a four-fold rise in titer. Best indication of immunity to poliovirus is the neutralization antibody test.

Reference Range:

PeaceHealth Laboratories