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Alias Names: Fetal RBC / Kleihauer Stain
Methodology: Staining of slides following acid elution of adult hemoglobin
Edit Date: 3/14/2009
Performed: Daily
Released: Same day
CPT Code: 85460
Specimen Collection Details
Collection: One 4 mL lavender top tube (EDTA) from mother. Also acceptable: bloody amniotic fluid or cerebrospinal fluid (CSF).
Handling: Send to the laboratory immediately; refrigerate if there will be a delay prior to testing.
Stability: 72 hours ambient or refrigerated.
Standard Volume: 4 mL EDTA whole blood.
Minimum Volume: 0.5 mL EDTA whole blood in a 1 mL microcollection tube.
Transport: Refrigerated.
Comments: The Kleihauer stain is useful to demonstrate and quantitate the presence of circulating fetal cells in maternal circulation. It is also useful to evaluate distribution of Hgb F in disorders such as hereditary persistence of fetal hemoglobin. Note: For post-partum maternal screening for possible Rh Immune Globulin (RhIG) dosing, the RhIG screen, Rh Immune Globulin Profile, Post-partum (52200), should be ordered. This panel uses a Fetal Screen assay, with quantitation of positive samples performed by Kleihauer staining. Optimal sensitivity to fetal-maternal hemorrhage occurs from samples collected shortly after delivery and tested as soon as possible.
Rejection Criteria: Frozen specimen; marked hemolysis.

< 0.1% fetal cells in maternal circulation.

PeaceHealth Laboratories