One 4 mL lavender top tube (EDTA). Fill tube at least 1/3 full.
Handling:
Refrigerate. Send to the laboratory within 24 hours.
Standard Volume:
4 mL EDTA whole blood.
Minimum Volume:
0.5 mL EDTA whole blood in a 1 mL microcollection tube.
Transport:
Refrigerated.
Comments:
Sodium metabisulfite-induced sickling of red cells containing hemoglobin S. The presence or absence of sickling is observed microscopically. This test becomes positive at Hgb S levels equal to or greater than 25%. Thus it is possible for a negative result to occur in some combined defects such as Hbg S accompanied by α or β thalassemia. For alternative or additional tests, see Hemoglobin S Quantitation (48830), or Hemoglobinopathy Evaluation (10570) .
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