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| 92590:HERPES SIMPLEX VIRUS (HSV) DNA, BY PCR | |||||||||
| Methodology: | Polymerase Chain Reaction (PCR) | ||||||||
| Edit Date: | 3/6/2008 | ||||||||
| Performed: | Daily | ||||||||
| Released: | 24-72 hours after setup at PeaceHealth Laboratories’ reference lab. | ||||||||
| CPT Code: | 87529-90 | ||||||||
| Specimen Collection Details | |||||||||
| Collection: | For specimens other than blood use Herpes Simplex Virus (HSV) by Real-Time Polymerase Chain Reaction (PCR) (58490). Collect one 5 mL red top tube. Also acceptable: One 7.5 mL serum separator tube (SST) or one 4 mL lavender top tube (EDTA). | ||||||||
| Handling: | Centrifuge and separate serum or plasma from cells and pour onto a plastic vial. Freeze specimen within 30 minutes of collection. Label ‘serum’ or ‘plasma’. | ||||||||
| Stability: | 8 hours ambient, 72 hours refrigerated, or 3 months frozen. | ||||||||
| Standard Volume: | 1 mL serum or plasma. | ||||||||
| Minimum Volume: | 0.5 mL serum or plasma. | ||||||||
| Transport: | Frozen on dry ice. | ||||||||
| Comments: | DNA deteriorates rapidly after 24 hours if not frozen. | ||||||||
| Rejection Criteria: | Heparinized or hemolyzed specimen; leaking container. | ||||||||
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Negative: HSV DNA not detected by PCR |
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| Positive: HSV DNA detected by PCR | |||||||||||||||||||