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Methodology: Complement Fixation (CF) / Immunodiffusion (ID)
Edit Date: 6/11/2010
Components: Histoplasma (ID) / Histoplasma mycelia (CF) / Histoplasma yeast (CF)
Performed: Monday-Friday
Released: 48 hours after setup at PeaceHealth Laboratories’ reference lab, next run if titered.
CPT Code: 86698-90 x3
Specimen Collection Details
Collection: One 7.5 mL serum separator tube (SST).
Handling: Allow to clot, centrifuge and immediately separate serum into a plastic vial. Label specimen clearly as ‘acute’ or ‘convalescent.’
Stability: Specimen stable 48 hours ambient, 14 days refrigerated, or 1 year frozen. Avoid repeated freeze/thaw cycles.
Standard Volume: 1 mL serum.
Minimum Volume: 250 µL serum.
Transport: Refrigerated.
Comments: Optimal specimen is frozen serum. Parallel testing is preferred and convalescent specimen must be received within 30 days from receipt of acute specimen. Label specimens clearly as ‘acute’ or ‘convalescent.’
Rejection Criteria: Plasma; severe lipemia; contaminated specimen; specimen exposed to repeated freeze/thaw cycles.

Histoplasma Antibody None detected
Histoplasma Yeast Antibody <1:8
Histoplasma Mycelia Antibody <1:8

PeaceHealth Laboratories