45270:LECITHIN/SPHINGOMYELIN RATIO & PHOSPHATIDYLGLYCEROL (L/S-PG)
Alias Names:
L/S-PG SA
Methodology:
Thin Layer Chromatography (TLC)
Edit Date:
1/3/2007
Performed:
Daily at 1300 (STAT at 800)
Released:
Results at 1700 (STAT at 1300)
CPT Code:
83661 / 84081
Specimen Collection Details
Collection:
5 mL amniotic fluid.
Handling:
Centrifuge for 3 minutes at 1700 rpm (lower than routine centrifuging). Transfer supernatant to a plastic, screw-cap vial and label ”spun.” Refrigerate. Include additional clinical information on requisition, i.e., weeks gestation, is patient diabetic, source if not amniotic fluid, e.g., vaginal pool.
Standard Volume:
5 mL amniotic fluid.
Minimum Volume:
3.5 mL amniotic fluid.
Transport:
Refrigerated.
Comments:
Visible meconium or blood greater than 2% Hct may interfere with the L/S ratio.