Print View Attached Docs:
Methodology: Indirect Fluorescent Antibody (IFA)
Edit Date: 6/11/2010
Performed: Monday-Friday
Released: 24 hours after setup at PeaceHealth Laboratories’ reference lab.
CPT Code: 86713-90
Specimen Collection Details
Collection: One 7.5 mL serum separator tube (SST).
Handling: Allow to clot, centrifuge and immediately separate serum from cells into a plastic vial. Label specimen as ‘acute’ or ‘convalescent.’
Stability: 48 hours ambient, 14 days refrigerated, or 1 year frozen. Avoid repeated freeze/thaw cycles.
Standard Volume: 1 mL serum.
Minimum Volume: 100 µL serum.
Transport: Refrigerated.
Rejection Criteria: Severely lipemic, contaminated, heat-inactivated, or hemolyzed specimen.

<1_16 IgM antibody to Legionella pneumophila serotypes 1-6 is measured using an IgM-specific conjugate. It is recommended that the IgM test always be performed in conjunction with IgG antibody test.

The IgM response to Legionella tends to develop concurrently with the IgG response and may remain elevated as long as the IgG response remains elevated. Cross-reactions have been described with several species of bacteria and Mycoplasma.

PeaceHealth Laboratories