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Alias Names: Borrelia Burgdorferi Antibodies Screen / Lyme Disease Antibodies
Methodology: Enzyme-Linked Immunosorbent Assay (ELISA)
Edit Date: 4/19/2010
Performed: Wednesday
Released: Same day as tested
CPT Code: 86618
Specimen Collection Details
Collection: One 7.5 mL serum separator tube (SST). Also acceptable: One 5 mL red top tube.
Handling: Allow to clot, centrifuge and separate serum from cells immediately and pour into a plastic vial. Refrigerate. Freeze at -20° C if specimen cannot be assayed within 72 hours. Avoid repeat freeze/thaw cycle. If multiple tests are ordered, send separate specimen for this test.
Standard Volume: 0.5 mL serum.
Minimum Volume: 200 µL serum.
Transport: Refrigerated, or frozen on dry ice.
Rejection Criteria: Gross lipemia or gross hemolysis.

Lyme Disease IgG and IgM Antibodies Result Interpretations, ELISA Method:
1.0 LIV
< 1.0 LIV
Negative: No significant level of detectable antibody to B. burgdorferi.
1.0 – 1.1 LIV
Equivocal: Specimen equivocal for antibodies to B. burgdorferi. If clinical symptoms warrant, suggest a Western Blot. Please notify PeaceHealth Laboratories within 14 days if you would like specimen sent for a Western Blot.
≥ 1.2 LIV
Positive: Presence of IgM and/or IgG antibody to B. burgdorferi. Suggest confirmation by Western Blot. Please notify PeaceHealth Laboratories within 14 days if you would like specimen sent for a Western Blot.
LIV = Lyme Index Value (Optical density of the patient sample).
Lyme disease IgM antibody levels usually peak 3-6 weeks after infection. IgG antibody levels begin to be detectable several weeks after infection. The IgG response may continue to develop over the course of several months and generally persists for years.

A negative result indicates that there was not serologic evidence of infection with B. burgdorferi at the time the specimen was collected. A negative result should not be the basis for excluding B. burgdorferi as the cause of illness, especially if blood was collected within 2 weeks of when symptoms began. If Lyme disease is strongly suspected, a second specimen should be collected 2-4 weeks after the first specimen and then tested.

A positive or equivocal result is presumptive evidence of the presence of anti-B. burgdorferi and should always be followed by second-step testing, i.e. Western Blot.

PeaceHealth Laboratories