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| 85310:LYME DISEASE, IgG & IgM BY WESTERN BLOT | |||||||||
| Alias Names: | Borrelia Burgdorferi | ||||||||
| Methodology: | Western Blot (WB) | ||||||||
| Edit Date: | 6/11/2010 | ||||||||
| Performed: | Sunday, Tuesday, Thursday, Friday | ||||||||
| Released: | 72 hours after setup at PeaceHealth Laboratories’ reference lab. | ||||||||
| CPT Code: | 86617-90 x2 | ||||||||
| Specimen Collection Details | |||||||||
| Collection: | One 7.5 mL serum separator tube (SST). | ||||||||
| Handling: | Allow to clot, centrifuge and separate serum from cells immediately. Refrigerate. | ||||||||
| Stability: | 48 hours ambient, 14 days refrigerated, or 1 year frozen. | ||||||||
| Standard Volume: | 1 mL serum. | ||||||||
| Minimum Volume: | 100 µL serum. | ||||||||
| Transport: | Refrigerated. | ||||||||
| Comments: | This test should be used for confirmation of an equivocal or positive Borrelia burgdorferi total antibodies, IgG and/or IgM test performed on patients less than four weeks after appearance of erythema migrans. | ||||||||
| Rejection Criteria: | Severely lipemic, hemolytic, or heat-inactivated specimen; CSF or plasma; specimen frozen/thawed more than once. | ||||||||
| Reference Range: | |||||||||||||||||||
| Negative. | |||||||||||||||||||
| IgG Positive |
Any five (5) of the following ten (10) bands: 18, 23, 28, 30, 39, 41, 45, 58, 66 or 93 kDa.
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| IgG Negative |
Any pattern that does not meet the IgG positive criteria.
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| IgM Positive |
Any two (2) of the following three (3) bands: 23, 39, 41 kDa.
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| IgM Negative |
Any pattern that does not meet the IgM positive criteria.
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| Per CDC guidelines, if ELISA test result is NEGATIVE, Western blot should not be performed. | |||||||||||||||||||