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| 33330:METHOTREXATE | |||||||||
| Methodology: | Fluorescence Polarization Immunoassay (FPIA) | ||||||||
| Edit Date: | 3/17/2009 | ||||||||
| Performed: | Daily | ||||||||
| Released: | Same day as tested | ||||||||
| CPT Code: | 80299 | ||||||||
| Specimen Collection Details | |||||||||
| Collection: | One 7.5 mL serum separator tube (SST). Also acceptable: One 5 mL red top tube, 4 mL green top tube (heparin), 4 mL lavender top tube (EDTA), or 2 mL grey top tube (sodium fluoride/potassium oxalate). | ||||||||
| Handling: | Protect specimen from light. Note time and date of last dose. Allow to clot, centrifuge and separate serum or plasma from cells within 2 hours. Freeze if specimen cannot be assayed within 24 hours. Allow no more than one freeze/thaw cycle. | ||||||||
| Stability: | 24 hours refrigerated. | ||||||||
| Standard Volume: | 0.5 mL serum or plasma. | ||||||||
| Minimum Volume: | 150 µL serum or plasma. | ||||||||
| Transport: | Refrigerated, or frozen on drdy ice. | ||||||||
| Comments: | The sampling time of methotrexate will depend on dose, duration of infusion, and clinical status of the patient. Consult Physicians’ Desk Reference (PDR) for specific treatment protocols. | ||||||||
| Rejection Criteria: | Specimen exposed to light; specimen frozen and thawed more than once; refrigerated specimen received in laboratory more than 24 hours after collection.
This test may require insurance company prior authorization before ordering.
Please check the prior authorization list . Failure to gain preauthorization may result in denial of coverage. |
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