33330:METHOTREXATE

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33330:METHOTREXATE
Methodology: Fluorescence Polarization Immunoassay (FPIA)
Edit Date: 3/17/2009
Performed: Daily
Released: Same day as tested
CPT Code: 80299
Specimen Collection Details
Collection: One 7.5 mL serum separator tube (SST). Also acceptable: One 5 mL red top tube, 4 mL green top tube (heparin), 4 mL lavender top tube (EDTA), or 2 mL grey top tube (sodium fluoride/potassium oxalate).
Handling: Protect specimen from light. Note time and date of last dose. Allow to clot, centrifuge and separate serum or plasma from cells within 2 hours. Freeze if specimen cannot be assayed within 24 hours. Allow no more than one freeze/thaw cycle.
Stability: 24 hours refrigerated.
Standard Volume: 0.5 mL serum or plasma.
Minimum Volume: 150 µL serum or plasma.
Transport: Refrigerated, or frozen on drdy ice.
Comments: The sampling time of methotrexate will depend on dose, duration of infusion, and clinical status of the patient. Consult Physicians’ Desk Reference (PDR) for specific treatment protocols.
Rejection Criteria: Specimen exposed to light; specimen frozen and thawed more than once; refrigerated specimen received in laboratory more than 24 hours after collection.
This test may require insurance company prior authorization before ordering.
Please check the prior authorization list
.
Failure to gain preauthorization may result in denial of coverage.

PeaceHealth Laboratories