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Methodology: Fluorescence Polarization Immunoassay (FPIA)
Edit Date: 3/17/2009
Performed: Daily
Released: Same day as tested
CPT Code: 80299
Specimen Collection Details
Collection: One 7.5 mL serum separator tube (SST). Also acceptable: One 5 mL red top tube, 4 mL green top tube (heparin), 4 mL lavender top tube (EDTA), or 2 mL grey top tube (sodium fluoride/potassium oxalate).
Handling: Protect specimen from light. Note time and date of last dose. Allow to clot, centrifuge and separate serum or plasma from cells within 2 hours. Freeze if specimen cannot be assayed within 24 hours. Allow no more than one freeze/thaw cycle.
Stability: 24 hours refrigerated.
Standard Volume: 0.5 mL serum or plasma.
Minimum Volume: 150 µL serum or plasma.
Transport: Refrigerated, or frozen on drdy ice.
Comments: The sampling time of methotrexate will depend on dose, duration of infusion, and clinical status of the patient. Consult Physicians’ Desk Reference (PDR) for specific treatment protocols.
Rejection Criteria: Specimen exposed to light; specimen frozen and thawed more than once; refrigerated specimen received in laboratory more than 24 hours after collection.
This test may require insurance company prior authorization before ordering.
Please check the prior authorization list
Failure to gain preauthorization may result in denial of coverage.

PeaceHealth Laboratories