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|Methodology:||Fluorescence Polarization Immunoassay (FPIA)|
|Released:||Same day as tested|
|Specimen Collection Details|
|Collection:||One 7.5 mL serum separator tube (SST). Also acceptable: One 5 mL red top tube, 4 mL green top tube (heparin), 4 mL lavender top tube (EDTA), or 2 mL grey top tube (sodium fluoride/potassium oxalate).|
|Handling:||Protect specimen from light. Note time and date of last dose. Allow to clot, centrifuge and separate serum or plasma from cells within 2 hours. Freeze if specimen cannot be assayed within 24 hours. Allow no more than one freeze/thaw cycle.|
|Stability:||24 hours refrigerated.|
|Standard Volume:||0.5 mL serum or plasma.|
|Minimum Volume:||150 µL serum or plasma.|
|Transport:||Refrigerated, or frozen on drdy ice.|
|Comments:||The sampling time of methotrexate will depend on dose, duration of infusion, and clinical status of the patient. Consult Physicians’ Desk Reference (PDR) for specific treatment protocols.|
|Rejection Criteria:||Specimen exposed to light; specimen frozen and thawed more than once; refrigerated specimen received in laboratory more than 24 hours after collection.
This test may require insurance company prior authorization before ordering.
Please check the prior authorization list .
Failure to gain preauthorization may result in denial of coverage.