Centrifuge for 3 minutes at 1700 rpm (lower than routine centrifuging). Transfer supernatant to a plastic screw-cap vial and label ”spun.” Refrigerate. Include additional clinical information on requisition, i.e., weeks gestation, is patient diabetic, source if not amniotic fluid, e.g., vaginal pool.
5 mL amniotic fluid.
1.5 mL amniotic fluid.
Visible meconium or blood are less likely to interfere, if present in moderate amounts.