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45300:PHOSPHATIDYLGLYCEROL (PG)
Alias Names:PG
Methodology:Thin Layer Chromatography (TLC)
Edit Date:4/1/2005
Performed:Daily at 0800 and 1200
Released:At 1200 and 1700
CPT Code:84081
Specimen Collection Details
Collection:5 mL amniotic fluid.
Handling:Centrifuge for 3 minutes at 1700 rpm (lower than routine centrifuging). Transfer supernatant to a plastic screw-cap vial and label ''spun.'' Refrigerate. Include additional clinical information on requisition, i.e., weeks gestation, is patient diabetic, source if not amniotic fluid, e.g., vaginal pool.
Standard Volume:5 mL amniotic fluid.
Minimum Volume:1.5 mL amniotic fluid.
Transport:Refrigerated.
Comments:Visible meconium or blood are less likely to interfere, if present in moderate amounts.
Rejection Criteria:Mucoid samples too viscous to pipette.