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Methodology: High Performance Liquid Chromatography (HPLC)
Edit Date: 8/2/2010
Performed: Monday-Friday
Released: 5 days after setup at PeaceHealth Laboratories’ reference lab.
CPT Code: 84311-90 / 82492-90 if reflexed
Specimen Collection Details
Collection: Two 4 mL green top tubes (sodium heparin). Also acceptable: Two 4 mL lavender top tubes (EDTA). Patient should be fasting 12-14 hours; water may be taken as needed. Patient should be off medication for 1 week and obstain from alcohol for 24 hours.
Handling: Centrifuge and separate plasma. Freeze in a plastic vial. Protect from light. If multiple tests are ordered, send separate specimen for this test.
Standard Volume: 3 mL plasma.
Minimum Volume: 1 mL plasma.
Transport: Frozen, on dry ice.
Rejection Criteria: Specimen other than heparin or EDTA plasma; specimen exposed to light.

Total porphyrins should not exceed 1.0 µg/dL
Component Reference Range
Uroporphyrins (octacarboxyl) ≤ 1.0 µg/dL
Heptacarboxylporphyrins ≤ 1.0 µg/dL
Hexacarboxylporphyrins ≤ 1.0 µg/dL
Pentacarboxylporphyrins ≤ 1.0 µg/dL
Coproporphyrins (tetracarboxyl) ≤ 1.0 µg/dL
Protoporphyrins ≤ 1.0 µg/dL
Includes separate quantitation of coproporphyrins, uroporphyrins, and intermediate porphyrins (heptacarboxyl, hexacarboxyl, pentacarboxyl, and tricarboxyl, when detected. Quantitation of a specific porphyrin in plasma can be helpful in establishing the diagnosis of a specific type of porphyria. In a case of chronic renal failure, analysis of the plasma porphyrins by type can be helpful in determining whether a porphyric patient has Porphyria cutanae tarda, due to a deficiency of uroporphyrinogen decarboxylase, or porphyria that is purely secondary to the failure of the normal renal excretion of porphyrinogens, mostly coproporphyrinogen.

This test is useful for the evaluation of persons with photosensitivity of the skin and blistering skin lesions, especially for cases of subjected variegate porphyria, coproporphyria and porphyria cutanae tarda. Also for persons in chronic renal failure who develop blistering skin lesions.

For at least 2 weeks prior to the test and under the guidance of the physician, medications should be avoided or minimized. Diabetics should detail what treatment protocol the physician prescribed during this same period. Abstinence from alcohol is essential. It is essential to proceed expeditiously with obtaining, processing, and dispatching the specimen, following precisely procedures and specifications and paying special heed to maintaining low temperatures.

PeaceHealth Laboratories