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Alias Names: AFB Culture & AFB Stain
Methodology: Continuous Monitoring Mycobacteria Culture System / Fluorescent Stain with Microscopic Exam
Edit Date: 6/11/2010
Performed: Daily
Released: Stain: Within 24 hours; Culture: Preliminary at 2 weeks or as soon as positive is detected. Final at 6 weeks.
CPT Code: 87015 / 87116 / 87206
Specimen Collection Details
Collection: Sputum, other respiratory specimens, urine, gastric aspirate, body fluid, cerebrospinal fluid (CSF), stool, bone marrow, or tissue. Collect sputum in sputum collection container; bronch brush in 2 mL sterile saline; all others in sterile plastic container. Add small amount of sterile saline to tissue. Gastric aspirate must be neutralized within 1 hour of collection to pH 7.0 using 100 mg of sodium carbonate. Swab and stool specimens are discouraged. If a swab is the only available specimen, submit in sterile container with 2-3 mL sterile saline. DO NOT use swab with transport media.
Stability: One hour ambient, or 72 hours refrigerated. Unstable frozen. It is recommended specimen be received in laboratory within 24 hours of collection.
Standard Volume: 5-10 mL respiratory; >50 mL urine; 5-10 mL gastric aspirate; 2-3 mL synovial fluid; 15-100 mL other body fluid; 2-3 mL CSF; 5-10 grams stool (5 mL if liquid); 0.5-2 mL bone marrow.
Minimum Volume: 3 mL respiratory; 10 mL urine; 5 mL gastric aspirate; 2 mL synovial fluid; 10 mL other body fluid; 2 mL CSF; 1 gm solid stool (1 mL if liquid); 0.5 mL bone marrow.
Transport: Refrigerated.
Comments: Please Note: If culture is for Mycobacterium genavense, specimen will be sent to a reference lab for culture. This organism will not grow on media routinely used for the isolation of mycobacteria. It is recommended that three sputum specimens be collected over an 8- to 24-hour time period, with at least one collected just after awakening. Three first-morning urine specimens should be collected on consecutive days. See also: Acid Fast Bacilli (AFB) Blood Culture (64500) for blood source. Acid fast bacilli which are isolated will be identified by DNA probe. Probe negative isolates will be sent to a reference laboratory for identification. Susceptibility testing will be done on all isolates of Mycobacterium tuberculosis. Additional charges may be added.
Rejection Criteria: Multiple same-site specimens collected in 24-hour period (sputum excepted); pooled sputum or urine; dry material; less than minimum volume received; delayed transport; specimen in EDTA; frozen specimen; rectal swab; un-neutralized gastric aspirate received in testing department more than one hour after collection; Note: purulent sputum specimen of 1-3 mL will be accepted with a qualifier. Specimen appearing to be saliva will be rejected regardless of volume. Leaking container will be rejected unless specimen is considered difficult to recollect.

Reference Range:
AFB Smear: No acid-fast bacilli seen.
AFB Culture: No acid-fast bacilli isolated at 6 weeks.

PeaceHealth Laboratories