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25050:PROTHROMBIN TIME (PT), HEPARIN NEUTRALIZED
Alias Names:Protime
Methodology:Mechanical Clot Detection following enzymatic neutralization of heparin
Edit Date:5/22/2009
Performed:Daily
Released:Same day
CPT Code:85525
Specimen Collection Details
Collection:One 4.5 mL light blue top tube (sodium citrate). Collect by careful venipuncture to avoid tissue contamination or hemolysis. Do not use needles smaller than 23 gauge. Fill tube to maximum draw, at least 75% full. Mix by gentle inversion. For hospitalized patients, indicate coumadin or heparin therapy. When drawing from heparin lines, withdraw slowly and discard at least 10 mL of blood to clear before drawing specimen.
Handling:Refrigerate and keep capped in original tube if specimen will be received at PeaceHealth Laboratories within 24 hours. If not, centrifuge to produce platelet-poor plasma (at least 1700 X G for 15 minutes) and transfer the top 2/3 of the plasma with a plastic pipette into a plastic vial, and freeze quickly. Label with patient name, test, and ''citrate.'' If multiple tests are ordered, freeze a separate aliquot for each test. Send to the laboratory within one week.
Stability:Unopened tube stable 24 hours refrigerated.
Opened tube stable 4 hours ambient, 4 hours refrigerated, or 7 days frozen.
Standard Volume:4.5 mL whole blood or 2 mL plasma.
Minimum Volume:1.8 mL whole blood or 0.5 mL plasma.
Transport:Original tube refrigerated if within 24 hours, or critrated plasma frozen on dry ice.
Comments:For hospitalized patients, indicate coumadin or heparin therapy. When drawing from heparin lines, withdraw slowly and discard at least 10 mL of blood to clear before drawing specimen.

Report includes PT result in seconds and INR.

See also: Coagulation Study, Prolonged Prothrombin Time (PT) Evaluation (25720).

The standard Prothrombin Time (PT) (25000) performed at PeaceHealth Laboratories contains a heparin neutralizing compound and is not affected by heparin up to levels of 2.0 U/mL. The ‘Heparin Neutralized PT’ (25050) is useful for Prothrombin Time evaluation in patients receiving high-dose heparin therapy.
Rejection Criteria:Serum; visible fibrin; moderate to marked hemolysis; improperly filled tube.
Reference Range:
Varies with reagent lot number. Current reference range included with report.