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Methodology: Enzyme-Linked Immunosorbent Assay (ELISA)
Edit Date: 4/19/2010
Performed: Tuesday, Friday
Released: Same day as tested
CPT Code: 86765
Specimen Collection Details
Collection: One 7.5 mL serum separator tube (SST). Also acceptable: One 5 mL red top tube.
Handling: Allow to clot, centrifuge and separate serum from cells immediately. Refrigerate. Feeze at -20 degrees C if specimen cannot be assayed within 48 hours. Allow no more than one freeze/thaw cycle. If multiple tests are ordered, send separate specimen for this test.
Standard Volume: 0.5 mL serum.
Minimum Volume: 200 µL serum.
Transport: Refrigerated if received in laboratory within 48 hours of collection, or frozen on dry ice.
Rejection Criteria: Gross lipemia; gross hemolysis; heat inactivated or icteric specimen; bacterial contamination; specimen frozen and thawed more than once; refrigerated specimen received in laboratory more than 48 hours after collection.

Rubeola (Measles) IgG Antibody Result Interpretation, ELISA Method:
<0.9 IV Negative: No significant level of detectable IgG Rubeola (Measles) antibody, presumed non-immune to measles infection.
0.9-1.0 IV Equivocal: Repeat testing in 10-14 days may be helpful.
>= 1.1 IV Positive: IgG antibody to Rubeola (Measles) detected, which may indicate a current or previous exposure or immunization to Rubeola (Measles). Positive IgG antibody levels in the absence of current clinical symptoms may indicate immunity.
IV = Index Value

The presence of specific antibodies in a single serum specimen indicates past measles infection or vaccination. Demonstration of a significant increase in antibody titers in a serum pair taken at a 14 day interval is the basis for diagnosis of acute infection.

PeaceHealth Laboratories