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| 58560:RUBEOLA (MEASLES) ANTIBODY, IgG | |||||||||
| Methodology: | Enzyme-Linked Immunosorbent Assay (ELISA) | ||||||||
| Edit Date: | 4/19/2010 | ||||||||
| Performed: | Tuesday, Friday | ||||||||
| Released: | Same day as tested | ||||||||
| CPT Code: | 86765 | ||||||||
| Specimen Collection Details | |||||||||
| Collection: | One 7.5 mL serum separator tube (SST). Also acceptable: One 5 mL red top tube. | ||||||||
| Handling: | Allow to clot, centrifuge and separate serum from cells immediately. Refrigerate. Feeze at -20 degrees C if specimen cannot be assayed within 48 hours. Allow no more than one freeze/thaw cycle. If multiple tests are ordered, send separate specimen for this test. | ||||||||
| Standard Volume: | 0.5 mL serum. | ||||||||
| Minimum Volume: | 200 µL serum. | ||||||||
| Transport: | Refrigerated if received in laboratory within 48 hours of collection, or frozen on dry ice. | ||||||||
| Rejection Criteria: | Gross lipemia; gross hemolysis; heat inactivated or icteric specimen; bacterial contamination; specimen frozen and thawed more than once; refrigerated specimen received in laboratory more than 48 hours after collection. | ||||||||
| Rubeola (Measles) IgG Antibody Result Interpretation, ELISA Method: | |||||||||||||||||||
| <0.9 IV | Negative: No significant level of detectable IgG Rubeola (Measles) antibody, presumed non-immune to measles infection. | ||||||||||||||||||
| 0.9-1.0 IV | Equivocal: Repeat testing in 10-14 days may be helpful. | ||||||||||||||||||
| >= 1.1 IV | Positive: IgG antibody to Rubeola (Measles) detected, which may indicate a current or previous exposure or immunization to Rubeola (Measles). Positive IgG antibody levels in the absence of current clinical symptoms may indicate immunity. | ||||||||||||||||||
| IV = Index Value | |||||||||||||||||||
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The presence of specific antibodies in a single serum specimen indicates past measles infection or vaccination. Demonstration of a significant increase in antibody titers in a serum pair taken at a 14 day interval is the basis for diagnosis of acute infection. |
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