40580:TESTOSTERONE, TOTAL & FREE

Author Modified by

Print View Attached Docs:
40580:TESTOSTERONE, TOTAL & FREE
Methodology: Chemiluminescent Immunoassay (CLIA) The concentration of free testosterone is derived from a mathematical expression based on constants for the binding of testosterone to sex hormone binding globulin.
Edit Date: 8/2/2008
Performed: Monday-Friday
Released: Same day
CPT Code: 84270 / 84403
Specimen Collection Details
Collection: One 7.5 mL serum separator tube (SST). Also acceptable: One 5 mL red top tube. Collect at least 4 mL. Draw specimen between 0600 and 1000 if possible, as testosterone levels vary throughout the day.
Handling: Allow to clot, centrifuge and separate serum from cells within 2 hours. Refrigerate. Freeze if specimen cannot be assayed within 7 days. Allow no more than one freeze/thaw cycle.
Stability: 7 days refrigerated at 2-8° C, or 2 months frozen at -20° C.
Standard Volume: 2 mL serum.
Minimum Volume: 1 mL serum.
Transport: Refrigerated, or frozen on dry ice.
Comments: Grossly lipemic specimens may yield falsely elevated values.
For testing on patients less than 1 year of age, see Testosterone, Total & Free, Women and Children (87135).
Rejection Criteria: Gross hemolysis; gross lipemia, unless cleared by ultracentrifugation; more than one freeze/thaw cycle.

Reference Range:
SERUM TOTAL TESTOSTERONE, ng/dL
Age Male Age Female
1-9 years <10 1-9 years <15
10-11 years <10-57 10-11 years <10-42
12-13 years <10-747 12-13 years <10-64
14-15 years 33-585 14-15 years <10-49
16-17 years 185-886 16-17 years <10-63
18-39 years 400-1080 18-40 years 15-70
40-59 years 350-890 41-51 years <10-70
≥ 60 years 350-720 Postmenopausal <10-50
Tanner Stage I <20 Tanner Stage I <17
Tanner Stage II <10-149 Tanner Stage II <10-39
Tanner Stage III <10-762 Tanner Stage III 10-60
Tanner Stage IV 164-854 Tanner Stage IV <10-63
Tanner Stage V 194-783 Tanner Stage V 10-60
FREE TESTOSTERONE, pg/mL
Age Male Age Female
1-9 years <1.0 1-9 years <1.8
10-11 years 0.1-6.3 10-11 years 0.1-3.5
12-13 years 0.5-98 12-13 years 0.9-6.8
14-15 years 3-138 14-15 years 1.2-7.5
16-17 years 38-173 16-17 years 1.2-9.9
≥ 18 years 47-244 18-30 years 0.8-7.4
31-40 years 1.3-9.2
41-51 years 1.1-5.8
Postmenopausal 0.6-3.8
Tanner Stage I <3.7 Tanner Stage I <2.2
Tanner Stage II 0.3-21 Tanner Stage II 0.4-4.5
Tanner Stage III 1.0-98 Tanner Stage III 1.3-7.5
Tanner Stage IV 35-169 Tanner Stage IV 1.1-15.5
Tanner Stage V 41-239 Tanner Stage V 0.8-9.2

PeaceHealth Laboratories
© 2023 PeaceHealth Laboratories