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Methodology: Nephelometry (NEPH) / Multiple Flow Immunoassay (Luminex)
Edit Date: 6/11/2010
Components: Antinuclear Antibodies (ANA) / Complement C3 / Complement C4 / Double Stranded DNA Antibody (dsDNA) / Extractable Nuclear Antigen (ENA) Antibodies, Sm & RNP / Extractable Nuclear Antigen (ENA) Antibodies, SS-A/Ro & SS-B/La
Performed: Daily
Released: Same day as tested
CPT Code: 86038 / 86160 x2 / 86225 / 86235 x4
Specimen Collection Details
Collection: Two 7.5 mL serum separator tubes (SST). Also acceptable: Two 5 mL red top tubes.
Handling: Allow to clot for 30-60 minutes at 20-25° C. Do not clot at 2-8° C. Centrifuge and within 1 hour separate serum from cells into two plastic vials.
For C3/C4 testing: Freeze one tube ≤ -20° C. Allow no more than one freeze/thaw cycle.
For ANA testing: Refrigerate second tube at 2-8° C. Freeze ≤ -20° C if specimen cannot be tested within 7 days. Allow no more than three freeze/thaw cycles.
Stability: C3/C4 testing:1 year frozen ≤ -20° C.
ANA testing: 7 days refrigerated at 2-8° C.
Standard Volume: 1 mL serum for ANA testing and 1 mL serum for C3/C4 testing.
Minimum Volume: 0.5 mL serum for C3/C4 testing and 0.5 mL serum for ANA testing.
Transport: Frozen on dry ice for C3/C4; refrigerated or frozen on dry ice for ANA testing.
Rejection Criteria: C3/C4 Testing: Plasma, grossly lipemic, hemolytic, or contaminated specimen; specimen allowed to clot at 2-8° C; specimen frozen/thawed more than once.
ANA Testing: Plasma; grossly lipemic, hemolytic, or contaminated specimen; refrigerated specimen received in laboratory more than 7 days after collection; specimen frozen/thawed more than 3 times.

See individual components.

PeaceHealth Laboratories