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| 10770:ANTINUCLEAR ANTIBODIES (ANA) PLUS ANA PROFILE | |||||||||
| Methodology: | Nephelometry (NEPH) / Multiple Flow Immunoassay (Luminex) | ||||||||
| Edit Date: | 6/11/2010 | ||||||||
| Components: | Antinuclear Antibodies (ANA) / Complement C3 / Complement C4 / Double Stranded DNA Antibody (dsDNA) / Extractable Nuclear Antigen (ENA) Antibodies, Sm & RNP / Extractable Nuclear Antigen (ENA) Antibodies, SS-A/Ro & SS-B/La | ||||||||
| Performed: | Daily | ||||||||
| Released: | Same day as tested | ||||||||
| CPT Code: | 86038 / 86160 x2 / 86225 / 86235 x4 | ||||||||
| Specimen Collection Details | |||||||||
| Collection: | Two 7.5 mL serum separator tubes (SST). Also acceptable: Two 5 mL red top tubes. | ||||||||
| Handling: | Allow to clot for 30-60 minutes at 20-25° C. Do not clot at 2-8° C. Centrifuge and within 1 hour separate serum from cells into two plastic vials. For C3/C4 testing: Freeze one tube ≤ -20° C. Allow no more than one freeze/thaw cycle. For ANA testing: Refrigerate second tube at 2-8° C. Freeze ≤ -20° C if specimen cannot be tested within 7 days. Allow no more than three freeze/thaw cycles. |
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| Stability: | C3/C4 testing:1 year frozen ≤ -20° C. ANA testing: 7 days refrigerated at 2-8° C. |
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| Standard Volume: | 1 mL serum for ANA testing and 1 mL serum for C3/C4 testing. | ||||||||
| Minimum Volume: | 0.5 mL serum for C3/C4 testing and 0.5 mL serum for ANA testing. | ||||||||
| Transport: | Frozen on dry ice for C3/C4; refrigerated or frozen on dry ice for ANA testing. | ||||||||
| Rejection Criteria: | C3/C4 Testing: Plasma, grossly lipemic, hemolytic, or contaminated specimen; specimen allowed to clot at 2-8° C; specimen frozen/thawed more than once. ANA Testing: Plasma; grossly lipemic, hemolytic, or contaminated specimen; refrigerated specimen received in laboratory more than 7 days after collection; specimen frozen/thawed more than 3 times. |
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| See individual components. | |||||||||||||||||||