Print View Attached Docs:
Methodology: Multiple Flow Immunoassay / Nephelometry (NEPH)
Edit Date: 6/11/2010
Components: Complement C3 / Complement C4 / Double Stranded DNA Antibody (DS DNA) / Extractable Nuclear Antigen (ENA) Antibodies, SM & RNP / Extractable Nuclear Antigen (ENA) Antibodies, SS-A/Ro & SS-B/La
Performed: Daily
Released: Same day as tested
CPT Code: 86225 / 86160 x2 / 86235 x4
Specimen Collection Details
Collection: Two 7.5 mL serum separator tubes (SST). Also acceptable: Two 5 mL red top tubes.
Handling: Allow to clot for 30-60 minutes at 20-25° C. Do not clot at 2-8° C. Centrifuge and within 1 hour separate serum into two plastic vials. For C3/C4 Testing: Freeze one tube ≤ -20°C. Allow no more than one freeze/thaw cycle. For ANA Testing: Refrigerate second tube at 2-8° C. Freeze ≤ -20° C if specimen cannot be tested within 7 days. Allow no more than three freeze/thaw cycles.
Stability: C3/C4: 1 year frozen ≤ -20° C; ANA: 7 days refrigerated or frozen ≤ -20°C for long-term storage.
Standard Volume: 1 mL serum for ANA testing and 1 mL serum for C3/C4 testing
Minimum Volume: 0.5 mL serum for ANA testing and 0.5 mL serum for C3/C4 testing.
Transport: C3/C4: Frozen on dry ice. ANA: Refrigerated or frozen on dry ice.
Rejection Criteria: C3/C4: Plasma; grossly lipemic, hemolytic, or contaminated specimen; specimen allowed to clot at 2-8° C; specimen frozen/thawed more than once. ANA: Plasma; grossly lipemic, hemolytic, or contaminated specimen; refrigerated specimen received in lab more than 7 days after collection; specimen frozen thawed more than 3 times

See individual components.

PeaceHealth Laboratories