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| 89220:VITAMIN B1 PYROPHOSPHATE (THIAMINE). WHOLE BLOOD | |||||||||
| Alias Names: | B1 / VIT B1 | ||||||||
| Methodology: | High Performance Liquid Chromatography (HPLC) for Thiochrome | ||||||||
| Edit Date: | 2/17/2010 | ||||||||
| Performed: | Daily | ||||||||
| Released: | 2-6 days after setup at PeaceHealth Laboratories’ reference lab. | ||||||||
| CPT Code: | 84425-90 | ||||||||
| Specimen Collection Details | |||||||||
| Collection: | One 4 mL green top tube (heparin). Do NOT use a plasma separator tube. Also acceptable: One 4 mL lavender top tube (EDTA) or 6 mL pink top tube (K2 EDTA). Protect from light during collection, storage, and transport. | ||||||||
| Handling: | Immediately transfer whole blood to a plastic vial and freeze. CRITICAL FROZEN. Protect from light. If multiple tests are ordered, send separate specimen for this test. | ||||||||
| Stability: | 4 hours refrigerated or 6 months frozen. Specimen unstable ambient. | ||||||||
| Standard Volume: | 3 mL whole blood. | ||||||||
| Minimum Volume: | 0.6 mL whole blood. | ||||||||
| Transport: | Frozen on dry ice. | ||||||||
| Rejection Criteria: | Specimen not frozen within 4 hours of collection; serum or plasma; gross lipemia; use of a PST; specimen exposed to light or submitted in glass tube; non-frozen or thawed specimen. | ||||||||
| 70-180 nmol/L
The concentration of thiamine diphosphate (TDP), the primary active form of vitamin B1, is measured in this assay. Approximately 90% of vitamin B1 present in whole blood is TDP. Thiamine and thiamine monophosphate, which comprise the remaining 10%, are not measured. Note: Whole blood is the preferred specimen for thiamine assessment. Approximately 80% of thiamine present in whole blood is found in red blood cells. This assay does not distinguish thiamine from its phosphate esters. |
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