89230:VITAMIN B6

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89230:VITAMIN B6
Methodology: High Performance Liquid Chromatography (HPLC)
Edit Date: 11/17/2010
Performed: Daily
Released: 1-4 days after setup at PeaceHealth Laboratories’ reference lab.
CPT Code: 84207-90
Specimen Collection Details
Collection: NOTE: Collect after an overnight fast. One 4 mL green top tube (heparin). Also acceptable: One 5 mL red top tube. Protect from light during collection, storage, and transport.
Handling: Allow to clot, centrifuge and immediately separate serum or plasma into a plastic vial. Protect from light during storage and transport. Freeze immediately. Critical frozen. Allow no more than one freeze/thaw cycle. If multiple tests are ordered, send separate specimen for this test.
Stability: After separation from cells specimen stable 4 hours refrigerated or 2 months frozen. Unstable ambient.
Standard Volume: 1 mL serum or plasma.
Minimum Volume: 0.5 mL serum or plasma.
Transport: Frozen on dry ice.
Comments: EDTA is not acceptable as a specimen collected in EDTA yields a higher pyridoxal 5-phosphate concentration.

Vitamin B6 deficiency causes an increase in xanthurenic acid in individuals ingesting sufficient tryptophan.

Rejection Criteria: Specimen frozen and thawed more than once; specimen not frozen within 4 hours of collection; EDTA; whole blood.

Adult: 5.0-30.0 ng/mL

PeaceHealth Laboratories