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| 89230:VITAMIN B6 | |||||||||
| Methodology: | High Performance Liquid Chromatography (HPLC) | ||||||||
| Edit Date: | 11/17/2010 | ||||||||
| Performed: | Daily | ||||||||
| Released: | 1-4 days after setup at PeaceHealth Laboratories’ reference lab. | ||||||||
| CPT Code: | 84207-90 | ||||||||
| Specimen Collection Details | |||||||||
| Collection: | NOTE: Collect after an overnight fast. One 4 mL green top tube (heparin). Also acceptable: One 5 mL red top tube. Protect from light during collection, storage, and transport. | ||||||||
| Handling: | Allow to clot, centrifuge and immediately separate serum or plasma into a plastic vial. Protect from light during storage and transport. Freeze immediately. Critical frozen. Allow no more than one freeze/thaw cycle. If multiple tests are ordered, send separate specimen for this test. | ||||||||
| Stability: | After separation from cells specimen stable 4 hours refrigerated or 2 months frozen. Unstable ambient. | ||||||||
| Standard Volume: | 1 mL serum or plasma. | ||||||||
| Minimum Volume: | 0.5 mL serum or plasma. | ||||||||
| Transport: | Frozen on dry ice. | ||||||||
| Comments: | EDTA is not acceptable as a specimen collected in EDTA yields a higher pyridoxal 5-phosphate concentration.
Vitamin B6 deficiency causes an increase in xanthurenic acid in individuals ingesting sufficient tryptophan. |
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| Rejection Criteria: | Specimen frozen and thawed more than once; specimen not frozen within 4 hours of collection; EDTA; whole blood. | ||||||||
| Adult: 5.0-30.0 ng/mL | |||||||||||||||||||