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Alias Names: Lymphocyte Immunodeficiency / T & B Lymphocyte Quantitation / B & T Lymphocyte Quantitation
Methodology: Flow Cytometry

Cell labelling with monoclonal antibody with analysis of lysed whole blood by flow cytometry. Antigenic sites on lymphocytes are tagged with monoclonal antibodies which have been labelled with fluorescent dye. This sample is then lysed, and the cells are individually passed through focused laser beams. Light scatter and fluorescence are then measured, and this data is used to enumerate the percentages of lymphocytes positive for the markers used.

Edit Date: 6/2/2010
Components: Total T Lymphocytes (CD3) / T Helper Lymphocytes (CD4) / T Suppressor Lymphocytes (CD8) / T Helper/Suppressor Ratio (CD4/8) / Total B Lymphocytes (CD19) / Total NK Lymphocytes (CD56)
Performed: Daily
Released: 24 hours
CPT Code: 86355 / 86356 / 86357 / 86359 / 86360
Specimen Collection Details
Collection: One 4 mL lavender top tube (EDTA).
Handling: Submit specimen to the laboratory within 48 hours.
Stability: 48 hours ambient or refrigerated. Unstable frozen.
Standard Volume: 4 mL whole blood.
Minimum Volume: 1 mL whole blood.
Transport: Ambient or refrigerated. If transport is delayed beyond 24 hours, perform CBC and submit results with specimen ambient or refrigerated.
Comments: This test is designed to evaluate B-cell, T-cell or NK-cell quantitative immunodeficiencies, including the most common inherited cellular immunodeficiency (combined variable immunodeficiency). For evaluation of possible lymphoma, CLL or other lymphoproliferative disorders see Flow Cytometry, Lymphoid Panel (29400).
Rejection Criteria: Clotted or frozen specimen; specimen more than 48 hours old at time of testing.

Relative Count Absolute Count
CD3 62.0-85.0% 850-2560/µL Total T Lymphocytes
CD4 39.0-64.0% 680-1760/µL T-Helper Lymphocytes
CD8 16.0-37.0% 200-900/uL T Suppressor/Cyclotoxic lymphs
CD19 5.0-22.0% 120-700/µL Total B Lymphocytes
CD19/27- 7.7-9.9% 65-244/µL Naïve B Lymphocytes


0.5-3.7% 10-63/µL Memory B Lymphocytes
CD19/27+/IgD 0.4-2.9% 6-54/µL Early Memory B Lymphocytes
CD56 3.0-18.0% 40-500/µL Totak NK Lymphocytes
CD 4/8 Ratio 1.0-3.2

PeaceHealth Laboratories