8 thick and 8 thin blood films prepared within 1 hour of collection from capillary puncture OR one 4 mL lavender top tube (EDTA) .
Refrigerate blood. Send slides in slide holders or wrap in heavy cardboard and tape closed.
8 thick and 8 thin smears OR 3 mL EDTA whole blood.
2 thick and 2 thin smears OR 1 mL EDTA whole blood.
Microscopic examination of Giemsa stained thick and thin blood preparations. EDTA whole blood may be used within 24 hours of collection; however, differentiation from malaria may be difficult in slides prepared from blood greater than 1 hour old.