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Methodology: Chemiluminescence
Edit Date: 1/6/2009
Performed: Daily
Released: Same day
CPT Code: 86762
Specimen Collection Details
Collection: One 7.5 mL serum separator tube (SST). Also acceptable: One 5 mL red top tube.
Handling: Allow to clot, centrifuge and separate serum from cells immediately. Refrigerate. Freeze if specimen cannot be assayed within 48 hours.
Standard Volume: 0.5 mL serum.
Minimum Volume: 150 µL serum.
Transport: Refrigerated; or if frozen, on dry ice.
Rejection Criteria: Gross hemolysis; gross lipemia; heat treated specimen; specimen frozen and thawed more than three times.

Reference Range:
<10.0 IU/mL Rubella IgG Quantitative Antibody Result Interpretation, Chemiluminescence Method:
<10.0 IU/mL
Negative: Non-immune, no significant level of detectable Rubella IgG antibody.
10.0-14.9 IU/mL
Equivocal: Repeat testing in 10-14 days may be helpful.
≥ 15.0 IU/mL
Positive: Immune, IgG antibody to Rubella detected, which may indicate a current or previous
exposure/immunization to Rubella.
IU/mL = International Units/mL

Seroconversion between acute and convalescent sera is considered strong evidence of current or recent infection. The best evidence for infection is a significant change on two appropriately timed specimens, where both tests are done in the same laboratory at the same time.

PeaceHealth Laboratories