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Methodology: Chemiluminescence (CL)
Edit Date: 4/18/2010
Components: Toxoplasma Antibody, IgG / Toxoplasma Antibody, IgM
Performed: Daily
Released: Same day as tested
CPT Code: 86777 / 86778
Specimen Collection Details
Collection: One 7.5 mL serum separator tube (SST). Also acceptable: One 5 mL red top tube.
Handling: Allow to clot, centrifuge and separate serum from cells within 2 hours. Refrigerate. Freeze if specimen cannot be assayed within 48 hours.
Stability: 48 hours refrigerated.
Standard Volume: 0.5 mL serum or plasma.
Minimum Volume: 150 µL serum or plasma.
Transport: Refrigerated; or if frozen, on dry ice.
Comments: This is a quantitative measurement of IgG antibodies to toxoplasma gondii in serum as an aid in determination of immune status. Toxoplasmosis is acquired by man via ingestion of cat feces or undercooked meats infested with cysts.

See also: TORCH Antibodies, IgG (58921) and TORCH Antibodies, IgG & IgM (58961).

Rejection Criteria: Gross hemolysis; gross lipemia; heat inactivation; bacterial contamination; specimen that has been frozen and thawed more than three times.

Toxoplasma IgG Antibody Result Interpretation, Chemilumiescent (CL)Method:
<6.5 IU/mL Negative: No significant level of antibody present. No exposure or infection is likely.
≥ 6.5-< 8.0 IU/mL Equivocal: Repeat testing in 10-14 days may be helpful.
≥ 8.0 IU/mL Positive: IgG antibody to Toxoplasma gondii detected, which may indicate acute or past infection.
Toxoplasma IgM Antibody Result Interpretation, Chemiluminescent (CL) Method:

IU/mL = International Units/mL

Seroconversion between acute and convalescent sera is considered strong evidence of current or recent infection. The best evidence for infection is a significant change on two appropriately timed specimens, where both tests are done in the same laboratory at the same time.

≤ 0.89 S/Co Negative: Non-reactive for IgM antibody to Toxoplasma gondii.
0.90-1.09 S/Co Equivocal: Repeat testing in 10-14 days may be helpful.
≥ 1.10 S/Co Positive: Reactive for IgM antibody to Toxoplasma gondii.

S/Co = Sample to Cut-off Ratio

While the presence of IgM antibodies suggests current or recent infection, low levels of IgM antibodies may occasionally persist for more than 12 months post-infection. Caution should be exercised in the use of IgM antibody levels in prenatal screening. Any positive results should be confirmed with amniocentesis and PCR testing for Toxoplasma gondii.

PeaceHealth Laboratories