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| 89200:VITAMIN A, SERUM | |||||||||
| Alias Names: | Retinol / Retinol Esters | ||||||||
| Methodology: | High Performance Liquid Chromatography (HPLC) | ||||||||
| Edit Date: | 6/11/2010 | ||||||||
| Components: | Retinyl Palmitate / vitamin A | ||||||||
| Performed: | Sunday, Tuesday-Saturday | ||||||||
| Released: | 1-4 days after setup at PeaceHealth Laboratories’ reference lab. | ||||||||
| CPT Code: | 84590-90 | ||||||||
| Specimen Collection Details | |||||||||
| Collection: | One 7.5 mL serum separator tube (SST). Also acceptable: One 4 mL green top tube (sodium heparin), or one 4 mL lavender top tube (EDTA). Draw following overnight (12 hour) fast. Patient must not consume alcohol for 24 hours prior to blood draw. Protect from light during collection, storage, and transport. | ||||||||
| Handling: | Allow to clot, centrifuge and separate serum or plasma from cells and pour into a plastic transport tube immediately. Protect from light. VERY LIGHT SENSITIVE. Refrigerate or freeze immediately. Specimen exposed to excessive light may show low results. | ||||||||
| Stability: | Specimen stable 1 month refrigerated or 1 year frozen. Specimen is unstable ambient. | ||||||||
| Standard Volume: | 1 mL serum or plasma. | ||||||||
| Minimum Volume: | 0.3 mL serum or plasma. | ||||||||
| Transport: | Refrigerated; or if frozen, on dry ice. | ||||||||
| Comments: | Serum retinol is typically maintained until hepatic stores are almost depleted. Values >0.20 mg/L probably represent adequate liver stores, whereas values < 0.10 mg/L may indicate severe deficiency. In acute hypervitaminosis A, retinol is often >18 mg/L and retinyl palmitate is often >5 mg/L.
Vitamin A supplements in the range of 10,000 IU/day are typically associated with retinyl palmitate <0.10 mg/L. Toxicity can occur when retinol exceeds the capacity of retinol binding protein (RBP); individuals with compromised renal function can retain RBP and may, therefore, have moderate retinol elevations. Drugs which interfere with vitamin A analysis include probucol (Lorelco). |
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| Rejection Criteria: | Hemolysis. | ||||||||
| Age | Reference Interval | ||||||||||||||||||
| Retinol (Vitamin A) | 0-1 month | 0.18-0.50 mg/L | |||||||||||||||||
| 2 months – 12 years | 0.20-0.50 mg/L | ||||||||||||||||||
| 13-17 years | 0.26-0.70 mg/L | ||||||||||||||||||
| 18 years/older | 0.30-1.2 mg/L | ||||||||||||||||||
| Retinyl Palmitate | 0.0-0.10 mg/L | ||||||||||||||||||