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Alias Names: Alpha Tocophero
Methodology: High Performance Liquid Chromatography (HPLC)
Edit Date: 6/11/2010
Performed: Sunday, Tuesday-Saturday
Released: 1-4 days after setup at PeaceHealth Laboratories’ reference lab.
CPT Code: 84446-90
Specimen Collection Details
Collection: One 7.5 mL serum separator tube (SST). Also acceptable: One 4 mL green top tube (heparin), or one 4 mL lavender top tube (EDTA). Draw following overnight (12 hour) fast. Patient must not consume alcohol for 24 hours prior to draw. Protect from light during collection, storage, and transport.
Handling: Allow to clot, centrifuge and separate serum or plasma from cells and pour into a plastic transport tube immediately. Refrigerate. Protect from light.
Stability: Specimen stable 1 month refrigerated or 1 year frozen. Specimen unstable ambient.
Standard Volume: 1 mL serum or plasma.
Minimum Volume: 200 µL serum or plasma.
Transport: Refrigerated.
Comments: Vitamin E often correlates with serum lipids. Significant deficiency of vitamin E (<3.0 mg/L) is associated with improper specimen collection and malabsorption syndromes. Significant excess (>40 mg/L) is often associated with hyperlipidemia.
Rejection Criteria: Hemolysis.

Alpha-tocopherol (Vitamin E) Interpretaton Gamma-Tocopherol (Vitamin E)
Age Premature infants 0-1 month 2-5 months 6 months – 1 year 2-12 years 13 years/older
Reference Range 0.5-3.5 mg/L 1.0-3.5 mg/L 2.0-6.0 mg/L 3.5-8.0 mg/L 5.5-9.0 mg/L 5.5-18.0 mg/L Normal 0.0-6.0 mg/L

PeaceHealth Laboratories