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Alias Names: AFB Stain Direct
Methodology: Fluorescent Stain with Microscopic Exam
Edit Date: 6/11/2010
Performed: Daily
Released: Final within 1 hour.
CPT Code: 87206
Specimen Collection Details
Collection: Sputum, other respiratory specimens, urine, gastric aspirate body fluid, cerebrospinal fluid (CSF), stool, bone marrow, or tissue. Collect sputum in a sputum collection container; bronch brush in 2 mL sterile saline; all others in sterile plastic container. Add a small amount of sterile saline to tissue. Gastric aspirate must be neutralized within 1 hour of collection to pH 7.0 using 100 mg sodium carbonate. Swab and stool specimens are discouraged. If a swab is the only available specimen, submit in sterile container with 2-3 mL sterile saline. DO NOT use swab with trasnport media.
Stability: One hour ambient, or 72 hours refrigerated Specimen unstable frozen. It is suggested specimen be received in laboratory within 24 hours of collection.
Standard Volume: 5-10 mL respiratory; >50 mL urine; 5-10 mL gastric aspirate; 2-3 mL synovial fluid; 15-100 mL other body fluid; 2-3 mL CSF; 5-10 grams stool (5 mL if liquid); 0.5-2 mL bone marrow.
Minimum Volume: 3 mL respiratory, 10 mL urine; 5 mL gastric aspirate; 2 mL synovial fluid; 10 mL other body fluid; 2 mL CSF; 1 grams stool (1 mL if liquid); 0.5 mL bone marrow.
Transport: Refrigerated.
Comments: It is recommended that three sputum specimens be collected over an 8- to 24-hour time period, with at least one collected just after awakening. Three first-morning urine specimens should be collected on consecutive days. Test is done STAT on unconcentrated specimens. If negative, test should be repeated on concentrated specimen order Acid Fast Bacilli (AFB) Culture & Acid Fast Bacilli (AFB) Stain (64450).
Rejection Criteria: Pooled sputum or urine; less than minimum volume received; dry material; delayed transport; specimen in EDTA; frozen specimen; rectal swab; less than minimum volume received. Note: purulent sputum specimen of 1-3 mL will be accepted with a qualifier. Specimen appearing to be saliva will be rejected regardless of volume. Leaking container will be rejected unless specimen is considered difficult to recollect.

No Acid Fast Bacilli seen.

PeaceHealth Laboratories