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Methodology: Enzyme-Linked Immunosorbent Assay (ELISA)
Edit Date: 5/18/2009
Performed: Varies
Released: 1-6 days after setup at PeaceHealth Laboratories’ reference lab.
CPT Code: 86790-90 x2
Specimen Collection Details
Collection: One 7.5 mL serum separator tube (SST).
Handling: Allow to clot, centrifuge and separate serum from cells and pour into a plastic vial with 10 minutes.
Stability: 5 days ambient, 14 days refrigerated, or 1 month frozen. Avoid repeated freeze/thaw cycles.
Standard Volume: 1 mL serum.
Minimum Volume: 250 µL serum.
Transport: Refrigerated.
Comments: Two major groups of hantaviruses are recognized based on clinical presentation. The first group includes Sin Nombre Virus, which causes hantavirus pulmonary syndrome, a severe and possibly fatal form of acute respiratory distress. A second group of hantaviruses (including Seoul, Hantaan, Dobrava, and Puumala viruses) causes hemorrhagic fever with renal syndrome, a condition not typically seen in the United States. Sera are initially screened for IgG and IgM antibodies recognizing the nucleocapsid protein common to all hantaviruses.

All screen IgM positive samples are then tested for SNV-specific IgM at an additional charge; further, any screen IgM positive samples that are also screen IgG positive are tested for SNV-specific IgG at an additional charge.

A positive result in the screening ELISA but a negative result in confirmatory assays may indicate reactivity to a hantavirus causing hemorrhagic fever with renal syndrome.

Rejection Criteria: Severely lipemic, contaminated, heat inactivated, or hemolyzed specimen; repeated freeze/thaw cycles.

<0.90 IV Negative: No significant level of detectable Hantavirus IgG/IgM antibodies.
0.90 – 1.10 IV Equivocal: Repeat testing in 10-14 days may be helpful.
>1.10 IV Positive: IgG/IgM antibodies to Hantavirus detected which may indicate a current or recent infection.
IV = Index Value

PeaceHealth Laboratories