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Alias Names: Rocky Mountain spotted fever / RMSF / Typhus-SLI
Methodology: Indirect Fluorescent Antibody (IFA)
Edit Date: 12/16/2004
Components: Rickettsia typhi IgG/IgM / Rickettsia rickettsii IgG/IgM
Performed: Tuesday, Thursday
Released: 1-5 days after setup at PeaceHealth Laboratories’ reference lab.
CPT Code: 86757-90 x4
Specimen Collection Details
Collection: One 7.5 mL serum separator tube (SST).
Handling: Allow to clot, centrifuge and separate serum from cells and pour into a plastic vial.
Stability: 1 hour ambient, 5 days refrigerated, or 2 months frozen.
Standard Volume: 1 mL serum.
Minimum Volume: 0.5 mL serum.
Transport: Refrigerated.
Comments: Paired testing of acute and convalescent specimens is recommended.
Helps establish the diagnosis of infection due to Rickettsia in the Rocky Mountain spotted fever group or in the typhus group. Reactivity to R. typhi antigen should be considered ‘group-reactive’ because the R. prowazekii infection also induces antibodies reactive with R. typhi.
Rejection Criteria: Plasma

Rickettsia Typhi IgG Antibodies
<1_64 titer
Rickettsia Typhi IgM Antibodies
<1_64 titer
Rickettsia Rickettsii IgG Antibodies
<1_64 titer
Rickettsia Rickettsii IgM Antibodies
<1_64 titer

PeaceHealth Laboratories