Print View Attached Docs:Comprehensive Thrombosis Panel Reconfigured (3/06)
Alias Names:Thrombosis Eval, Pregnant Patient
Methodology:Polymerase Chain Reaction (PCR) / Enzyme-Linked Immunosorbent Assay (ELISA) / Mechanical Clot Detection
Edit Date:6/6/2008
Components:Activated Protein C Resistance / Anticardiolipin Antibodies, IgG & IgM / Beta-2 Glycoprotein Antibodies, IgG & IgM / Lupus Anticoagulation / Protein C Activity / Prothrombin Gene Mutation
CPT Code:83891-3G / 83896-3G x2 / 83898-3G / 83903-3G / 83912-3G / 85303 / 85307 / 85613 / 85730 / 86146 x2 / 86147 x2
Specimen Collection Details
Collection:Total of five tubes collected: Three 4.5 mL light blue top tubes (sodium citrate) AND one 5 mL lavender top tube (EDTA) AND one 5 mL red top tube or serum separator tube (SST). Collect by careful venipuncture to avoid tissue fluid contamination or hemolysis. Do not use needles smaller than 23 guage. Fill blue tubes to maximum draw (at least 90% full). Mix by gentle inversion. Call the Special Coagulation Department (541-687-2134 x4612) when urgent testing is needed in order to assure STAT testing.
Handling:Because of short stability, specimens must either be sent STAT to arrive at the laboratory within 4 hours, or sent frozen. For STAT, refrigerate and keep capped in the original tubes. Call for STAT pick-up. For standard delivery, centrifuge blue tubes to produce platelet-poor plasma (at least 1700 X g for 15 minutes) and using a plastic pipette, transfer the top 2/3 of the plasma into 3-4 plastic vials. Freeze immediately. Label citrated plasma with patient name, I.D. number, test, and “citrated plasma.” Serum from red top tube can be centrifuged and transferred to a single tube, labeled with patient name, I.D. number, test, and “serum.” The EDTA tube must be submitted as refrigerated unopened whole blood. Specimens should be sent to the laboratory within 7 days. If multiple tests are ordered, freeze a separate aliquot for this test.
Stability:Original tubes: 4 hours refrigerated; citrated plasma: 7 days frozen; whole blood: 7 days refrigerated
Standard Volume:Three 4.5 mL light blue top tubes (or 4 mL plasma), one 5 mL red top tube (or 1 mL serum), and one 4 mL lavender top tube whole blood.
Minimum Volume:One 4.5 mL light blue top tube (or 2 mL plasma) and 1 mL lavender top tube whole blood.
Transport:Original tubes refrigerated if sent STAT; citrated plasma frozen on dry ice, whole blood and serum refrigerated.
Rejection Criteria:Frozen whole blood; markedly hemolyzed specimen; specimen received in laboratory beyond the stated stability.