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90640:PROTOPORPHYRINS, FRACTIONATION, RBC
High-Performance Liquid Chromatography (HPLC) with Fluorometric End Point
72 hours after setup at PeaceHealth Laboratories’ reference lab. Not reported Saturday or Sunday.
Specimen Collection Details
One 4 mL green top tube (heparin). Also acceptable: One 4 mL lavender top tube (EDTA). Patient must be fasting 12-14 hours and abstain from alcohol for 24 hours.
Place on wet ice immediately. Transfer blood to a 12 mL graduated centrifuge tube. Centrifuge for 10 minutes at 2000 rpm. Record volume of packed cells. Discard supernatant plasma. Wash erythrocytes three times by resuspension in 5 mL of cold 0.9% saline. Resuspend packed cells to original volume with 0.9% saline and freeze in a plastic vial. Allow no more than one freeze/thaw cycle. Volume of packed cells and total volume of erythrocyte suspension (red cells + saline) are required for testing.
Frozen. Unstable ambient or refrigerated.
4 mL red cell resuspension.
1 mL red cell resuspension.
Frozen on dry ice.
Specimen frozen and thawed more than once; specimen other than heparin or EDTA; frozen whole blood.
Includes separate quantitation of zinc protoporphyrins and free protoporphyrins.
<20 µg/dL packed cells
<60 µg/dL packed cells
Values of zinc-complexed protoporphyrin >60 ug/dL suggest iron deficiency anemia or may indicate chronic intoxication by heavy metals or organic chemicals. A predominance of noncomplexed (free) protoporphyrin indicates a diagnosis of EPP.
PeaceHealth Laboratories, formerly Oregon Medical Laboratories &
Northwest Regional Laboratory. All rights reserved.