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| 90640:PROTOPORPHYRINS, FRACTIONATION, RBC | |||||||||
| Methodology: | High-Performance Liquid Chromatography (HPLC) with Fluorometric End Point | ||||||||
| Edit Date: | 2/11/2010 | ||||||||
| Performed: | Monday-Friday | ||||||||
| Released: | 72 hours after setup at PeaceHealth Laboratories’ reference lab. Not reported Saturday or Sunday. | ||||||||
| CPT Code: | 82491-90 | ||||||||
| Specimen Collection Details | |||||||||
| Collection: | One 4 mL green top tube (heparin). Also acceptable: One 4 mL lavender top tube (EDTA). Patient must be fasting 12-14 hours and abstain from alcohol for 24 hours. | ||||||||
| Handling: | Place on wet ice immediately. Transfer blood to a 12 mL graduated centrifuge tube. Centrifuge for 10 minutes at 2000 rpm. Record volume of packed cells. Discard supernatant plasma. Wash erythrocytes three times by resuspension in 5 mL of cold 0.9% saline. Resuspend packed cells to original volume with 0.9% saline and freeze in a plastic vial. Allow no more than one freeze/thaw cycle. Volume of packed cells and total volume of erythrocyte suspension (red cells + saline) are required for testing. | ||||||||
| Stability: | Frozen. Unstable ambient or refrigerated. | ||||||||
| Standard Volume: | 4 mL red cell resuspension. | ||||||||
| Minimum Volume: | 1 mL red cell resuspension. | ||||||||
| Transport: | Frozen on dry ice. | ||||||||
| Rejection Criteria: | Specimen frozen and thawed more than once; specimen other than heparin or EDTA; frozen whole blood. | ||||||||
| Reference Range: | |||||||||||||||||||
| Includes separate quantitation of zinc protoporphyrins and free protoporphyrins. | |||||||||||||||||||
| Free Protoporphyrin | <20 µg/dL packed cells | ||||||||||||||||||
| Zinc-Complexed Protoporphyrin | <60 µg/dL packed cells | ||||||||||||||||||
| Values of zinc-complexed protoporphyrin >60 ug/dL suggest iron deficiency anemia or may indicate chronic intoxication by
heavy metals or organic chemicals. A predominance of noncomplexed (free) protoporphyrin indicates a diagnosis of EPP. |
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