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| 87080:ALKALINE PHOSPHATASE ISOENZYMES | |||||||||
| Alias Names: | Phosphatase Isoenzymes | ||||||||
| Methodology: | Heat Inactivation / Enzymatic | ||||||||
| Edit Date: | 6/11/2010 | ||||||||
| Performed: | Daily | ||||||||
| Released: | 24-72 hours after setup at PeaceHealth Laboratories’ reference lab. | ||||||||
| CPT Code: | 84080-90 | ||||||||
| Specimen Collection Details | |||||||||
| Collection: | One 7.5 mL serum separator tube (SST). Also acceptable: One 4 mL green top tube (heparin). Fasting specimen is recommended. | ||||||||
| Handling: | Allot to clot, centrifuge and separate serum from cells immediately and pour into a plastic vial. Freeze. Allow no more that one freeze/thaw cycle. | ||||||||
| Stability: | After separation specimen stable 1 hour ambient, 7 days refrigerated (total activity will increase 2% per day), or 1 year frozen. Allow no more than one freeze/thaw cycle. | ||||||||
| Standard Volume: | 2 mL serum or plasma. | ||||||||
| Minimum Volume: | 1 mL serum or plasma. | ||||||||
| Transport: | Frozen on dry ice. | ||||||||
| Comments: | This test cannot be added on at a later date due to stability.
Heat-stable isoenzymes, such as the one produced during pregnancy, will interfere with the interpretation of this assay. |
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| Rejection Criteria: | EDTA, sodium fluoride/potassium oxalate plasma, or hemolyzed specimen. | ||||||||
| Bone | Male | Female | |||||||||||||||||
| 1-6 years:0-208 U/L | 1-6 years: 0-189 U/L | ||||||||||||||||||
| 7-9 years: 0-264 U/L | 7-9 years: 0-246 U/L | ||||||||||||||||||
| 10-15 years: 0-340 U/L | 10-13 years: 0-340 U/L | ||||||||||||||||||
| 16-19 years: 0-165 U/L | 14-15 years: 0-91 U/L | ||||||||||||||||||
| 20 years >:0-55 U/L | 16 years >: 0-55 U/L | ||||||||||||||||||
| Liver | 1-6 years: 0-145 U/L | 1-9 years: 0-148 U/L | |||||||||||||||||
| 7-11 years: 0-182 U/L | 10-15 years: 0-162 U/L | ||||||||||||||||||
| 12-15 years: 0-226 U/L | 16 years >: 0-94 U/L | ||||||||||||||||||
| 16-19 years: 0-114 U/L | |||||||||||||||||||
| 20 years >: 0-94 U/L | |||||||||||||||||||
| Alkaline Phosphatase, Serum or Plasma | Age | Male | Female | ||||||||||||||||
| 0-30 days | 60-320 U/L | 60-320 U/L | |||||||||||||||||
| 1-12 months | 70-350 U/L | 70-350 U/L | |||||||||||||||||
| 1-3 years | 125-320 U/L | 125-320 U/L | |||||||||||||||||
| 4-6 years | 125-320 U/L | 150-370 U/L | |||||||||||||||||
| 7-9 years | 150-370 U/L | 150-440 U/L | |||||||||||||||||
| 10-11 years | 150-470 U/L | 150-530 U/L | |||||||||||||||||
| 12-13 years | 160-500 U/L | 110-525 U/L | |||||||||||||||||
| 14-15 years | 130-530 U/L | 55-305 U/L | |||||||||||||||||
| 16-19 years | 60-270 U/L | 40-120 U/L | |||||||||||||||||
| 20> years | 40-120 U/L | 40-120 U/L | |||||||||||||||||