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Methodology:Fluorescence Polarization Immunoassay (FPIA)
Edit Date:3/6/2008
Components:Primidone and Phenobarbital.
Released:24 hours after setup at PeaceHealth Laboratories’ reference lab
CPT Code:80188-90 / 80184-90
Specimen Collection Details
Collection:One 5 mL red top tube. Also acceptable: One 4 mL green top tube (heparin). Do not use serum separator tube (SST) or gels.
Handling:Allow to clot at ambient temperature, centrifuge and separate 1 mL serum or plasma from cells and pour into plastic vial immediately. Refrigerate.
Stability:7 days ambient, 3 weeks refrigerated, or 6 months frozen.
Standard Volume:1 mL serum or plasma.
Minimum Volume:0.8 mL serum or plasma.
Comments:This test includes phenobarbital. Do not order phenobarbital separately.
Rejection Criteria:Use of serum separator tube (SST) or gels.
Primidone (Mysoline) 5.0-12.0 µg/mL
0-2 months 15.0-30.0 µg/mL
0-2 months toxic level >40.0 µg/mL
>=3 months 15.0-40.0 µg/mL
>=3 months toxic level >50.0 µg/mL