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| 58300:EXTRACTABLE NUCLEAR ANTIGEN (ENA) ANTIBODIES, SS-A/Ro & SS-B/La | |||||||||
| Alias Names: | Anti-ENA Antibodies, SS-A & SS-B, Anti-SS-A/Ro, Anti-SS-B/La, ENA II, Sjogren Antibodies, SS-A & SS-B, SS-A & SS-B | ||||||||
| Methodology: | Multiplex Flow Immuniassay (Luminex) | ||||||||
| Edit Date: | 4/19/2010 | ||||||||
| Performed: | Daily | ||||||||
| Released: | Same day as tested | ||||||||
| CPT Code: | 86235 x2 | ||||||||
| Specimen Collection Details | |||||||||
| Collection: | One 7.5 mL serum separator tube (SST). Also acceptable: One 5 mL red top tube. | ||||||||
| Handling: | Allow to clot, centrifuge and immediately separate serum into a plastic vial. Refrigerate. Freeze ≤-20° C if specimen cannot be assayed within 7 days. Allow no more than three freeze/thaw cycles. | ||||||||
| Stability: | 8 hours ambient, 7 days refrigerated 2-8° C. Freeze ≤-20° C for longer storage. | ||||||||
| Standard Volume: | 1 mL serum. | ||||||||
| Minimum Volume: | 0.5 mL serum. | ||||||||
| Transport: | Refrigerated or frozen on dry ice. | ||||||||
| Rejection Criteria: | Plasma; grossly lipemic, hemolytic, or contaminated specimen; specimen received in laboratory more than 8 hours ambient, or 7 days refrigerated, after collection; specimen frozen/thawed more than 3 times. | ||||||||
| Reference Range: | |||||||||||||||||||
| <1.0 AI (Antibody Index: Relative Fluorescent Intensity) | |||||||||||||||||||
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Antibodies to SS-A(Ro) have been deteced in apppoximately 25-30% of patients with Systemic Lupus Erythematosus (SLE) and 40-70% of patients with Sjogren’s Syndrome. In addition, anti-SS-A(Ro) antibodies have been associated with homozygous complement C2 and C4 deficiencies, subacute cutaneous lupus erythematosus and neonatal lupus. SS-B(La) antibody is seen in 50-60% of Sjogren’s syndrome and is specific if it is the only ENA antibody present. 15-20% of patients with Systemic Lupus Erythematosus (SLE) and 5-10% of patients with progressive systemic sclerosis also have this antibody. |
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