Print View Attached Docs:New BioPlex 2200 Antinuclear Antibodies (ANA) Testing
Alias Names: Anti-ENA Antibodies, SS-A & SS-B, Anti-SS-A/Ro, Anti-SS-B/La, ENA II, Sjogren Antibodies, SS-A & SS-B, SS-A & SS-B
Methodology: Multiplex Flow Immuniassay (Luminex)
Edit Date: 4/19/2010
Performed: Daily
Released: Same day as tested
CPT Code: 86235 x2
Specimen Collection Details
Collection: One 7.5 mL serum separator tube (SST). Also acceptable: One 5 mL red top tube.
Handling: Allow to clot, centrifuge and immediately separate serum into a plastic vial. Refrigerate. Freeze ≤-20° C if specimen cannot be assayed within 7 days. Allow no more than three freeze/thaw cycles.
Stability: 8 hours ambient, 7 days refrigerated 2-8° C. Freeze ≤-20° C for longer storage.
Standard Volume: 1 mL serum.
Minimum Volume: 0.5 mL serum.
Transport: Refrigerated or frozen on dry ice.
Rejection Criteria: Plasma; grossly lipemic, hemolytic, or contaminated specimen; specimen received in laboratory more than 8 hours ambient, or 7 days refrigerated, after collection; specimen frozen/thawed more than 3 times.

Reference Range:
<1.0 AI (Antibody Index: Relative Fluorescent Intensity)

Antibodies to SS-A(Ro) have been deteced in apppoximately 25-30% of patients with Systemic Lupus Erythematosus (SLE) and 40-70% of patients with Sjogren’s Syndrome. In addition, anti-SS-A(Ro) antibodies have been associated with homozygous complement C2 and C4 deficiencies, subacute cutaneous lupus erythematosus and neonatal lupus.

SS-B(La) antibody is seen in 50-60% of Sjogren’s syndrome and is specific if it is the only ENA antibody present. 15-20% of patients with Systemic Lupus Erythematosus (SLE) and 5-10% of patients with progressive systemic sclerosis also have this antibody.

PeaceHealth Laboratories