58250:EXTRACTABLE NUCLEAR ANTIGEN (ENA) ANTIBODIES, SM & RNP
ANTI-ENA Antibodies / ANTI-RNP / ANTI-SM / ENA I / SM & RNP Antibodies
Multiplex Flow Immunoassay (Luminex)
Same day as tested
Specimen Collection Details
One 7.5 mL serum separator tube (SST). Also acceptable: One 5 mL plain red top tube.
Allow to clot, centrifuge and immediately separate serum into a plastic vial. Refrigerate. Freeze ≤ – 20° C if specimen cannot be assayed within 7 days. Allow no more than three freeze/thaw cycles.
8 hours ambient or 7 days refrigerated at 2-8° C. Freeze ≤ -20° C for long-term storage.
1 mL serum.
0.5 mL serum.
Refrigerated, or frozen on dry ice.
Plasma; grossly lipemic, hemolytic, or contaminated specimen; specimen frozen and thawed more than three times; specimen received in laboratory more than 8 hours ambient, or more than 7 days refrigerated, after collection.
<1.0 AI (Antibody Index: Relative Fluorescent Intensity)
Sm (Smith) Antibody is specific for Systemic Lupus Erythematosus (SLE) but only occurs in 30-35% of cases, these are often associated with renal disease.
RNP (Ribonucleic Protein) Antibody is seen in 95-100% of mixed connective tissue disease and is considered specific for this syndrome if other antibodies are negative; RNP is also present in 20-30% of Systemic Lupus Erythematosus (SLE) and 15-20% of progressive system sclerosis.