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| 69170:NEUTROPHIL CYTOPLASMIC ANTIBODY (ANCA) SCREEN, REFLEX CONFIRMATION IF POSITIVE | |||||||||
| Alias Names: | Anti-Neutrophil Cytoplasmic Antibody | ||||||||
| Methodology: | Indirect Fluorescent Antibody (IFA) | ||||||||
| Edit Date: | 6/11/2010 | ||||||||
| Performed: | Monday, Wednesday, Friday | ||||||||
| Released: | Same day as tested | ||||||||
| CPT Code: | 86255 | ||||||||
| Specimen Collection Details | |||||||||
| Collection: | One 7.5 mL serum separator tube (SST). Also acceptable: One 5 mL red top tube. | ||||||||
| Handling: | Allow to clot, centifuge and separate serum from cells immediately and pour into a plastic vial. Refrigerate until transport. Freeze at -20° C if specimen cannot be assayed within 48 hours. Avoid repeated freeze/thaw cycles. | ||||||||
| Standard Volume: | 1.5 mL serum. | ||||||||
| Minimum Volume: | 1 mL serum. | ||||||||
| Transport: | Refrigerated or frozen on dry ice. | ||||||||
| Rejection Criteria: | Plasma, gross lipemia or hemolysis, contaminated or heat inactivated specimen; specimen frozen/thawed more than three times. | ||||||||
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<1_20 titer
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Neutrophil Cytoplasmic Antibody (ANCA) Screen Result Interpretation, IFA Method:
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<1_20 titer
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Negative: No significant level of Anti-Neutrophil Cytoplasmic Antibody (ANCA) detected.
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≥ 1:20 titer
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Positive: Anti-Neutrophil Cytoplasmic Antibody (ANCA) detected.
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| Samples are screened on ethanol fixed slides which allow differentiation of C and P patterns. Negative results are reported. Positive results are serially diluted and tested on ethanol fixed slides to establish titer and confirm pattern. All P-ANCA patterns are also tested on formalin fixed slides to confirm P-ANCAs by removing Anti-Nuclear Antibody (ANA) receptor sites and maintaining cytoplasmic localization of Myeloperoxidase or other antigens. Positive ANCA Screen results will reflex to ANCA Titer (69175) and Neutrophil Cytoplasmic Antibody (ANCA) MPO/PR3 Confirmation (69171) if needed. Additional charges will apply. |
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