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| 45000:BILIRUBIN & OXYHEMOGLOBIN SCAN, CEREBROSPINAL FLUID | |||||||||
| Alias Names: | Xanthochromia / Delta OD450 | ||||||||
| Methodology: | Spectrophotometry (S) | ||||||||
| Edit Date: | 6/11/2010 | ||||||||
| Performed: | Monday-Friday | ||||||||
| Released: | Next day | ||||||||
| CPT Code: | 82247 | ||||||||
| Specimen Collection Details | |||||||||
| Collection: | Cerebrospinal fluid (CSF) in a sterile container. | ||||||||
| Handling: | Centrifuge and separate supernatant into new sterile tube within 1 hour of collection. Label tube as supernatant. Protect from light by wrapping in foil. Refrigerate. | ||||||||
| Standard Volume: | 2 mL CSF. | ||||||||
| Minimum Volume: | 1.5 mL CSF. | ||||||||
| Transport: | Refrigerated. | ||||||||
| Comments: | Examination for xanthochromia requires that CSF be centrifuged within one hour or less after collection to avoid false positives since initial lysis of RBCs begins after one to four hours.
About 2-4 hours after subarachnoid hemorrhage, oxyhemoglobin appears in CSF, peaking at 24-36 hours and disappearing at 4-8 days. About 12 hours after subarachnoid hemorrhage, bilirubin appears in CSF, peaking at about 2-4 days and gradually disappearing at 2-4 weeks. |
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| Rejection Criteria: | Supernatant not protected from light or not separated within 1 hour of collection. | ||||||||