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Methodology: Culture
Edit Date: 6/11/2010
Performed: Daily
Released: Preliminary at 1 week. Final negative 4 weeks. Final positive > 4 weeks.
CPT Code: 87101
Specimen Collection Details
Collection: Skin scrapings, nail scrapings, or hair in a clean, dry plastic container. Skin: Clean the infected area with 70% alcohol. Gently scrape the surface of the skin at the active margin of the lesion with the edge of a sterile scalpel, sterile tongue blade, or the edge of a sterile slide. Send scalpel, tongue blade, or slide along with the scrapings. Hair: With forceps, collect at least 10-12 affected hairs with the base of the shaft intact. For hairs broken off at scalp level, use the edge of a scalpel or knife blade to remove the shaft. Collect scalp scales, if present, along with scrapings of active borders of lesions. Nails: Wipe nail(s) with 70% alcohol. Remove outermost layer by scraping with a scalpel and discard these scrapings. Collect deeper scrapings and material from under the nail for culture.
Stability: 72 hours ambient. Specimen unstable refrigerated or frozen.
Transport: Ambient. .
Comments: Each isolate of mould or yeast will be identified. An additional charge will be added for each identification. See also: Fungus Culture & KOH Preparation – Skin, Hair, or Nails (64310) or Fungus Culture (64300).
Rejection Criteria: Delayed transport; refrigerated or frozen specimen.

Reference Range:
No yeast or filamentous fungi isolated at 4 weeks.

PeaceHealth Laboratories