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| 64310:FUNGUS CULTURE & KOH PREPARATION – SKIN, HAIR, or NAILS | |||||||||
| Methodology: | Culture | ||||||||
| Edit Date: | 6/11/2010 | ||||||||
| Performed: | Culture: Daily; KOH: Monday-Friday | ||||||||
| Released: | Culture: Preliminary at 1 week. Final negative at 4 weeks. Final positive > 4 weeks. KOH: 24 hours (if received Monday-Friday). |
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| CPT Code: | 87101 / 87220 | ||||||||
| Specimen Collection Details | |||||||||
| Collection: | Skin scrapings, nail scrapings, or hair in a clean, dry plastic container. Skin: Clean the infected area with 70% alcohol. Gently scrape the surface of the skin at the active margin of the lesion with the edge of a sterile scalpel, sterile tongue blade, or the edge of a sterile slide. Send scalpel, tongue blade, or slide along with the scrapings. Hair: With forceps, collect at least 10-12 affected hairs with the base of the shaft intact. For hairs broken off at scalp level, use the edge of a scalpel or knife blade to remove the shaft. Collect scalp scales, if present, along with scrapings of active borders of lesions. Nails: Wipe nail(s) with 70% alcohol. Remove outermost layer by scraping with a scalpel and discard these scrapings. Collect deeper scrapings and material from under the nail for culture. | ||||||||
| Stability: | 72 hours ambient. Unstable refrigerated or frozen. | ||||||||
| Transport: | Ambient. | ||||||||
| Comments: | Each isolate of mould or yeast will be identified. An additional charge will be added for each identification. If Actinomadura, Dermatophilus, Gordonia, Nocardia, Rhodococcus, Streptomyces, or Tsukamurella are suspected, order Actinomycetes Culture, Aerobic (60160). See also: Fungus Culture, Skin, Hair, or Nails (64302) or Fungus Culture (64300). | ||||||||
| Rejection Criteria: | Delayed transport; refrigerated or frozen specimen. | ||||||||
| Reference Range: | |||||||||||||||||||
| KOH: | No yeast or mycelial elements seen. | ||||||||||||||||||
| Fungus Culture: | No yeast or filamentous fungi isolated at 4 weeks. | ||||||||||||||||||