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Methodology: Culture
Edit Date: 4/13/2010
Performed: Daily
Released: Preliminary at 24 and 48 hours. Final at 72 hours.
CPT Code: 87045 / 67046 x3
Specimen Collection Details
Collection: Stool in enteric transport (C&S) medium. Acceptable for infant: Rectal specimen on Routine Culture transport swab. No more than one specimen should be collected in a 24-hour period.
Stability: C&S medium 24 hours ambient or 48 hours refrigerated. Transport swab 24 hours ambient or refrigerated. All specimen types unstable frozen. NOTE: Stool NOT in C&S medium will be accepted only if received ambient and within two hours of collection, and delivered STAT to PeaceHealth Laboratories’ Microbiology department.
Standard Volume: 5 gm solid stool or 5 mL liquid stool.
Minimum Volume: 2 gm solid stool or 2 mL liquid stool.
Transport: Ambient or refrigerated.
Comments: Brochure provided with C&S medium contains instructions for collection. For diagnostic testing: Ideally, 2-3 specimens should be collected on consecutive days, however, specimens collected after the third day of hospital stay are of limited value. Testing for Clostridium difficile should be considered as an alternative to culture in all patients over the age of 6 months with clinically significant diarrhea and a history of antibiotic exposure. A biochemical screen may be done when presumptive pathogens are isolated. If indicated, identification will follow. Additional charges will be added for this test.

This test detects the presence of Salmonella, Shigella, E. coli 0157:H7, Campylobacter, Yersinia pseudotuberculosis and Yersinia enterocolitica. Additional media for the isolation of Vibrio will be included by request.

Rejection Criteria: Delayed transport; rectal swab with no visible fecal material; swab not in transport media; specimen mixed with water or urine; frozen specimen; more than one specimen collected within a 24-hour period.

Reference Range:
No Salmonella or Shigella isolated.
No E.coli 0157:H7 isolated.
No Campylobacter isolated.
No Yersinia isolated.

PeaceHealth Laboratories