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|81620:BORDETELLA PERTUSSIS SMEAR|
|Alias Names:||Pertussis OFA|
|Methodology:||Direct Fluorescent Antibody stain (DFA)|
|Released:||Within 24 hours after setup at PeaceHealth Laboratories’ reference lab; positive stains are reported as soon as detected.|
|Specimen Collection Details|
|Collection:||Nasopharynx swab, nasal washing, or aspirate (moist swab or prepared slide).|
|Handling:||Leave swab in Regan-Lowe and incubate 24-48 hours at 35° C , or send without incubation. Send two prepared slides or moist swab in bacterial transport media (Regan-Lowe, or Aimes with or without charcoal). Specimen source required.|
|Stability:||Specimen: 48 hours ambient or 7 days refrigerated. Unstable frozen.
Slides: 7 days ambient, 7 days refrigerated, or 1 month frozen.
Regan-Lowe: 4 days ambient or 4 days refrigerated. Unstable frozen.
|Comments:||Culture, in addition to DFA, is suggested. Client is notified of positive stain/culture.|
|Rejection Criteria:||Improper labeling of slide for DFA; dry swab; sputum; specimen in M4 medium.|
|Negative for B pertussis.|