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Alias Names: Pertussis OFA
Methodology: Direct Fluorescent Antibody stain (DFA)
Edit Date: 6/11/2010
Performed: Daily
Released: Within 24 hours after setup at PeaceHealth Laboratories’ reference lab; positive stains are reported as soon as detected.
CPT Code: 87265-90
Specimen Collection Details
Collection: Nasopharynx swab, nasal washing, or aspirate (moist swab or prepared slide).
Handling: Leave swab in Regan-Lowe and incubate 24-48 hours at 35° C , or send without incubation. Send two prepared slides or moist swab in bacterial transport media (Regan-Lowe, or Aimes with or without charcoal). Specimen source required.
Stability: Specimen: 48 hours ambient or 7 days refrigerated. Unstable frozen.

Slides: 7 days ambient, 7 days refrigerated, or 1 month frozen.

Regan-Lowe: 4 days ambient or 4 days refrigerated. Unstable frozen.

Transport: Ambient.
Comments: Culture, in addition to DFA, is suggested. Client is notified of positive stain/culture.
Rejection Criteria: Improper labeling of slide for DFA; dry swab; sputum; specimen in M4 medium.

Negative for B pertussis.

PeaceHealth Laboratories