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| 81620:BORDETELLA PERTUSSIS SMEAR | |||||||||
| Alias Names: | Pertussis OFA | ||||||||
| Methodology: | Direct Fluorescent Antibody stain (DFA) | ||||||||
| Edit Date: | 6/11/2010 | ||||||||
| Performed: | Daily | ||||||||
| Released: | Within 24 hours after setup at PeaceHealth Laboratories’ reference lab; positive stains are reported as soon as detected. | ||||||||
| CPT Code: | 87265-90 | ||||||||
| Specimen Collection Details | |||||||||
| Collection: | Nasopharynx swab, nasal washing, or aspirate (moist swab or prepared slide). | ||||||||
| Handling: | Leave swab in Regan-Lowe and incubate 24-48 hours at 35° C , or send without incubation. Send two prepared slides or moist swab in bacterial transport media (Regan-Lowe, or Aimes with or without charcoal). Specimen source required. | ||||||||
| Stability: | Specimen: 48 hours ambient or 7 days refrigerated. Unstable frozen.
Slides: 7 days ambient, 7 days refrigerated, or 1 month frozen. Regan-Lowe: 4 days ambient or 4 days refrigerated. Unstable frozen. |
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| Transport: | Ambient. | ||||||||
| Comments: | Culture, in addition to DFA, is suggested. Client is notified of positive stain/culture. | ||||||||
| Rejection Criteria: | Improper labeling of slide for DFA; dry swab; sputum; specimen in M4 medium. | ||||||||
| Negative for B pertussis. | |||||||||||||||||||