81670:BORRELIA BURGDORFERI, IgG & IgM BY EIA and IB

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81670:BORRELIA BURGDORFERI, IgG & IgM BY EIA and IB
Alias Names: B. Burgdorferi / Lyme Disease
Methodology: Enzyme Immunoassay (EIA) / Immunoblot (IB)
Edit Date: 3/12/2008
Performed: Tuesday-Saturday
Released: 1-5 days after setup at PeaceHealth Laboratories’ reference lab.
CPT Code: 86617-90 x2 / 86618-90 x2
Specimen Collection Details
Collection: One 7.5 mL serum separator tube (SST).
Handling: Allow to clot, centrifuge and separate serum from cells and pour into a plastic vial.
Stability: 7 days ambient , 14 days refrigerated, or 2 months frozen.
Standard Volume: 1 mL serum.
Minimum Volume: 0.5 mL serum.
Transport: Refrigerated.
Comments: Following the two-tier test algorithm proposed by the CDC for assessment of exposure to B. burgdorferi during early- and late-stage disease, both EIA IgM and IgG antibody responses to B. burgdorferi in human serum, are confirmed using an immunoblot (IB) method where whole-cell sonicate (WCS) B. burgdorferi antigens are utilized in identifying B. burgdorferi specific antigen binding reactivity. There are multiple criteria for interpreting Lyme immunoblots. The CDC recommends that at least 2 of 3 IgM bands (23, 39, or 41 kDa) must be present for interpretation of an IgM positive blot. For interpretation of an IgG positive blot, at least 5 of 10 IgG bands (18, 23, 28, 30, 39, 41, 45, 58, 66, or 93kDa) must be present. Only positive and negative interpretations are reported.

Component Reference Range
Borrelia burgdorferi IgG Antibodies – IB Negative
Borrelia burgdorferi IgM Antibodies – IB Negative
Borrelia burgdorferi IgG Antibodies – EIA < .80 Index
Borrelia burgdorferi IgM Antibodies – EIA < .80 Index

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