81670:BORRELIA BURGDORFERI, IgG & IgM BY EIA and IB
Alias Names:
B. Burgdorferi / Lyme Disease
Methodology:
Enzyme Immunoassay (EIA) / Immunoblot (IB)
Edit Date:
3/12/2008
Performed:
Tuesday-Saturday
Released:
1-5 days after setup at PeaceHealth Laboratories’ reference lab.
CPT Code:
86617-90 x2 / 86618-90 x2
Specimen Collection Details
Collection:
One 7.5 mL serum separator tube (SST).
Handling:
Allow to clot, centrifuge and separate serum from cells and pour into a plastic vial.
Stability:
7 days ambient , 14 days refrigerated, or 2 months frozen.
Standard Volume:
1 mL serum.
Minimum Volume:
0.5 mL serum.
Transport:
Refrigerated.
Comments:
Following the two-tier test algorithm proposed by the CDC for assessment of exposure to B. burgdorferi during early- and late-stage disease, both EIA IgM and IgG antibody responses to B. burgdorferi in human serum, are confirmed using an immunoblot (IB) method where whole-cell sonicate (WCS) B. burgdorferi antigens are utilized in identifying B. burgdorferi specific antigen binding reactivity. There are multiple criteria for interpreting Lyme immunoblots. The CDC recommends that at least 2 of 3 IgM bands (23, 39, or 41 kDa) must be present for interpretation of an IgM positive blot. For interpretation of an IgG positive blot, at least 5 of 10 IgG bands (18, 23, 28, 30, 39, 41, 45, 58, 66, or 93kDa) must be present. Only positive and negative interpretations are reported.
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