25100:ACTIVATED PARTIAL THROMBOPLASTIN TIME (APTT)

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25100:ACTIVATED PARTIAL THROMBOPLASTIN TIME (APTT)
Alias Names: Partial Thromboplastin Time / PTT / APTT / Anticoagulant Monitoring,Argatroban / Unfractionated Heparin / Hirudin/Lepirudin/Bivalirudin
Methodology: Mechanical Clot Detection
Edit Date: 4/24/2009
Performed: Daily
Released: Same day
CPT Code: 85730
Specimen Collection Details
Collection: One 4.5 mL light blue top tube or two 2.7 mL light blue top tubes (sodium citrate). Collect by careful venipuncture to avoid tissue fluid contamination or hemolysis. Do not use needles smaller than 23 gauge. Fill tube to maximum draw (at least 90% full). Mix by gentle inversion. When drawing from a heparin line, withdraw slowly and discard at least 10 mL of blood to clear before drawing specimen. If patient HCT is >55%, collection into an adjusted volume of anticoagulant is required. Call the Coagulation Department (541.687.2134 x4665) for instructions.
Handling: Due to short stability, specimen must either be sent STAT to reach the testing department within 4 hours, or sent frozen. For STAT, submit whole blood in original unopened tube. Call for STAT pick-up. For standard transport, centrifuge to produce platelet poor plasma (at least 1700 X G for 15 minutes) and transfer using a plastic pipette, the top 2/3 of the plasma into a plastic vial. Label with patient name, I.D. number, test and “citrate plasma” and freeze immediately. Send to the laboratory within 7 days. If multiple tests are ordered, freeze a separate aliquot for this test.
Stability: 4 hours ambient or refrigerated, or 7 days frozen.
Standard Volume: 5 mL whole blood or 2 mL plasma.
Minimum Volume: 3 mL whole blood or 0.5 mL plasma.
Transport: Original unopened tube refrigerated if STAT, or citrated plasma frozen on dry ice in a Styrofoam container.
Comments: For hospitalized patients, indicate coumarin or heparin therapy. Heparin interferes with most clotting assays.
Rejection Criteria: Serum; visible fibrin; moderate to marked hemolysis; specimen received in testing department more than 4 hours after collection.
MEDICARE MAY REQUIRE A SIGNED ADVANCE BENEFICIARY NOTICE (ABN) FOR THIS TEST

Reference Range:
Varies with reagent lot number. Current reference range included with report. Heparin therapeutic range is determined for each reagent lot, and can be obtained by contacting the Coagulation Department.