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|86295:MUMPS VIRUS ANTIBODY, IgM|
|Methodology:||Enzyme-Linked Immunosorbent Assay (ELISA)|
|Performed:||Monday, Wednesday, Friday|
|Released:||1-6 days after setup at PeaceHealth Laboratories’ reference lab.|
|Specimen Collection Details|
|Collection:||One 7.5 mL serum separator tube (SST).|
|Handling:||Allow to clot, centrifuge and immediately separate serum from cells . Label specimen as ”acute” or ”convalescent.”|
|Stability:||48 hours ambient, 14 days refrigerated, or 1 year frozen. Avoid repeated freeze/thaw cycles.|
|Standard Volume:||1 mL serum.|
|Minimum Volume:||200 µL serum.|
|Comments:||Parallel testing is preferred and convalescent specimen MUST be received within 30 days from receipt of acute specimen.|
|Rejection Criteria:||Severely lipemic, or hemolyzed specimen; heat-inactivated serum; contaminated specimen.|
|IV = Index Value|
≤ 0.79 IV – Negative: No significant level of detectable IgM antibody to Mumps virus.
0.80-1.20 IV – Equivocal: Borderline levels of IgM anti body to Mumps virus. Repeat testing in 10-14 days may be helpful.
≥ 1.21 IV – Positive: Presence of IgM antibody to Mumps virus detected and may indicate a current or recent infection. However, low levels of IgM anti body may occasionally persist for more than 12 months post-infection or immunization.