81080:LACTATE DEHYDROGENASE (LDH), ISOENZYMES
Author PeaceHealth Laboratories
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| Methodology: |
Enzymatic/Electrophoresis (EP) |
| Edit Date: |
6/11/2010 |
| Performed: |
Daily |
| Released: |
24-48 hours after setup at PeaceHealth Laboratories’ reference lab. |
| CPT Code: |
83625-90 / 83615-90 |
| Specimen Collection Details |
| Collection: |
One 7.5 mL serum separator tube (SST). |
| Handling: |
Allow to clot completely at ambient temperature. Separate serum from cells immediately. |
| Stability: |
7 days ambient. Unstable refrigerated or frozen. |
| Standard Volume: |
1 mL serum. |
| Minimum Volume: |
0.6 mL serum. |
| Transport: |
Ambient. |
| Comments: |
Do not freeze or refrigerate. Hemolyzed specimens and serum which have not been separated from cells show elevated LD-1 and LD-2. LD-4 and LD-5 are labile at low temperatures and are erroneously low in samples that have been frozen. |
| Rejection Criteria: |
Frozen, refrigerated or hemolyzed specimen; specimen collected with EDTA or potassium oxalate/sodium fluoride anticoagulants. |
|
|
LD-1: |
14-27% |
|
LD-2: |
29-42% |
|
LD-3: |
16-22% |
|
LD-4: |
8-15% |
|
LD-5: |
6-23% |
|
|
|
|
0 up to 1 mo: |
200-465 U/L |
|
1 mo-17 mos: |
200-450 U/L |
|
18 mos-10 yrs: |
165-430 U/L |
|
11 yrs-16 yrs: |
135-310 U/L |
|
17 yrs & over: |
105-230 U/L |
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