27500:CARDIOLIPIN ANTIBODIES, IgG & IgM

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27500:CARDIOLIPIN ANTIBODIES, IgG & IgM
Alias Names: Anti-Cardiolipin Antibodies / Anti-Phospholipid Antibodies
Methodology: Enzyme-Linked Immunosorbent Assay (ELISA)
Edit Date: 4/19/2010
Performed: Monday, Thursday
Released: Same day as tested
CPT Code: 86147 x2
Specimen Collection Details
Collection: One 5 mL red top tube. Also acceptable: One 7.5 mL serum separator tube (SST).
Handling: Allow to clot, centrifuge and separate serum from cells immediately and pour into an aliquot tube. Refrigerate. Freeze at -20° C if specimen can not be assayed within 48 hours.
Standard Volume: 2 mL serum.
Minimum Volume: 0.5 mL serum.
Transport: Refrigerated or frozen on dry ice.
Comments: See also: Coagulation Study, Anti-Phospholipid Panel (25620) .
Rejection Criteria: Refrigerated specimen received in laboratory more than 48 hours after collection; plasma; microbially contaminated, heat-treated, grossly hemolyzed or lipemic specimen.

Reference Ranges:
Result Interpretations, ELISA Method:
Cardiolipin, IgG <16 GPL Normal
17-25 GPL Borderline
26-79 GPL Positive
≥ 80 GPL High Positive
Cardiolipin, IgM <12 MPL Normal
12-19 MPL Borderline
20-79 MPL Positive
≥ 80 MPL High Positive
GPL = IgG Phospholipid Units
MPL = IgM Phospholipid Units

Studies suggest that the higher the IgG anticardiolipin antibody titer, the greater the clinical significance. Values greater than 40 may be the most significant based on recent studies, however an absolute threshold has not been clearly established. Results in the borderline range and isolated elevations of IgM are of uncertain clinical significance and must be carefully interpreted with regard to the clinical picture. Transient positive results occur, usually in association with infection. Demonstrated persistence of positive lab findings (retest after 8 weeks) is required for the lab diagnosis of anti-phospholipid syndrome. Other phospholipid-directed antibodies (e.g., anti-beta2 glycoprotein1, Beta2 Glycoprotein1 Antibodies IgG & IgM, 86990) may be useful in some patients.