48001:IMMUNOFIXATION, SERUM (includes Protein Electrophoresis)

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48001:IMMUNOFIXATION, SERUM (includes Protein Electrophoresis)
Alias Names: IFE, Serum / IEP Serum / Immuno Serum
Methodology: Capillary Zone Electrophoresis
Edit Date: 12/8/2010
Components: Immunofixation, Serum / Protein Electrophoresis
Performed: Monday-Friday
Released: 1-3 days of receipt in lab
CPT Code: 86334 / 84165
Specimen Collection Details
Collection: One 7.5 mL serum separator tube (SST). Also acceptable: One 5 mL red top tube.
Handling: Allow to clot, centrifuge and separate serum from cells within 4 hours. Refrigerate.
Stability: 7 days refrigerated, or 30 days frozen.
Standard Volume: 1 mL serum.
Minimum Volume: 0.7 mL serum.
Transport: Refrigerated.
Comments: This test includes a serum protein electrophoresis. Do not order Protein Electrophoresis, Serum (48010), additionally. See also Protein Electrophoresis with IFE if Indicated (48019)
Rejection Criteria: Plasma.

Reference Range:
Component Reference Range %
Protein, Total 6.2-8.4 g/dL
Albumin 3.5-5.0 g/dL 55-75%
Alpha-1 0.1-0.3 g/dL 3-6%
Alpha-2 0.3-1.0 g/dL 5-13%
Beta-1 0.3-0.7 g/dL 4-9%
Beta-2 0.1-0.4 g/dL 2-5%
Gamma 0.6-1.6 g/dL 9-20%