80880:SINGLE STRANDED DNA ANTIBODY

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80880:SINGLE STRANDED DNA ANTIBODY
Alias Names: Anti-DNA Antibody, Single Stranded / Anti-ssDNA Antibldy, IgG
Methodology: Enzyme-Linked Immunossorbent Assay (ELISA)
Edit Date: 6/11/2010
Performed: Tuesday, Thursday, Saturday.
Released: 1-4 days after setup at PeaceHealth Laboratories’ reference lab.
CPT Code: 86226-90
Specimen Collection Details
Collection: One 7.5 mL serum separator tube (SST).
Handling: Allow to clot, centrifuge and immediately separate serum from cells into a plastic vial.
Stability: 48 hours ambient, 14 days refrigerated, or 1 year frozen. Avoid repeated freeze/thaw cycles.
Standard Volume: 1 mL serum
Minimum Volume: 100 µL serum
Transport: Refrigerated
Comments: The ssDNA antibody test detects ssDNA and some dsDNA antibodies. As a result, patient samples should also be tested for dsDNA antibodies in order to more accurately determine the level of anti-ssDNA antibodies. By calculating the ratio (ssDNA/dsDNA), a conclusion can be made as to the presence of ssDNA antibodies. A ratio less than or equal to 1.0 indicates the absence of ssDNA antibodies. A ratio greater than 1.0 indicates the presence of ssDNA antibodies.
Rejection Criteria: Plasma; severly lipemic, contaminated, or hemolyzed specimen.

<20 EU Normal
20-25 EU Borderline positive
>25 EU Positive
EU=Enzyme Units