60682:FLUID IN A BOTTLE CULTURE AND SMEAR
Author PeaceHealth Laboratories
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| Methodology: |
Continuous Monitoring Bacterial System and Microscopic Exam |
| Edit Date: |
12/30/2009 |
| Performed: |
Daily. |
| Released: |
Preliminary at 24 hours or as soon as positive is detected. Final at 5 days. |
| CPT Code: |
87070 / 87075 / 87205 |
| Specimen Collection Details |
| Collection: |
2 specimens: 1) CULTURE: 5-10 mL body fluid in a BacT/Alert FA (aerobic) bottle AND a FN (anaerobic) bottle using aseptic technique. Bottles must be at ambient temperature. Inspect broth and sensor. Do not use if broth is cloudy or sensor is yellow. 2) GRAM STAIN: Submit specimen in sterile container. Accepted but not recommended: One sterile 4 mL green top tube (heparin). Do not refrigerate or freeze. |
| Stability: |
24 hours ambient. Unstable refrigerated or frozen. |
| Standard Volume: |
Specimen 1) BacT/Alert FA & FN bottles: 10 mL fluid each. Specimen 2) 2 mL fluid. |
| Minimum Volume: |
Specimen 1) BacT/Alert FA & FN bottles: 1 mL fluid each. Specimen 2) 1 mL fluid. |
| Transport: |
Ambient. |
| Comments: |
Includes aerobic and anaerobic cultures. Potential pathogens will be identified. Susceptibility testing will be done if appropriate. Additional charges will be added for these tests. See also: Fluid in a Bottle Culture (60691). |
| Rejection Criteria: |
Delayed transport; frozen specimen. |
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Gram Stain |
No organisms seen |
|
Culture |
No growth at 5 days |
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