80852: Mycoplasma Pneumoniae Antibody, IgG & IgM

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80852:MYCOPLASMA PNEUMONIAE ANTIBODY, IgG & IgM
Methodology: Enzyme-Linked Immunosorbent Assay (ELISA)
Edit Date: 6/11/2010
Performed: Daily
Released: 24-72 hours after setup at PeaceHealth Laboratories’ reference lab, next run if titered.
CPT Code: 86738-90 x2
Specimen Collection Details
Collection: One 7.5 mL serum separator tube (SST).
Handling: Allow to clot, centrifuge and separate serum from cells and immediately pour into a plastic vial. Refrigerate. Specimens must be labeled ‘acute’ or ‘convalescent’.
Stability: 48 hours ambient, 14 days refrigerated, or 1 year frozen.
Standard Volume: 0.5 mL serum.
Minimum Volume: 100 µL serum.
Transport: Refrigerated.
Comments: Parallel testing is preferred and convalescent specimen must be received within 30 days of the receipt of acute specimen.
Rejection Criteria: Plasma; severly lipemic, hemolyzed, heat-inactivated, contaminated, or icteric specimen.

Reference Range:
Mycoplasma pneumoniae Antibody, IgG <0.10 U/L Negative
0.10-0.32 U/L Equivocal
>0.32 /L Positive
Mycoplasma pneumoniae Antibody, IgM ≤0.76 U/L Negative. No clinically significant amount of M. pneumoniae antibody detected.
0.77-0.95 U/L Low positive. M. pneumoniae-specific IgM presumptively detected. Collection of a follow-up sample in one to two weeks is recommended to assure reactivity.
≥0.96 U/L Positive. Highly significant amount of M. pneumoniae-specific IgM antibody detected. However, low levels of IgM antibodies may occasionally persist for more than 12 months post-infection.